Interactive/automated method to count bacterial colonies

Ribeiro,  J.E.; Martins, Ramiro; Monteiro, Fernando C.

Publication

Interactive/automated method to count bacterial colonies

2015Conference object

dc.contributor.author	Ribeiro, J.E.
dc.contributor.author	Martins, Ramiro
dc.contributor.author	Monteiro, Fernando C.
dc.date.accessioned	2018-04-06T13:17:04Z
dc.date.available	2018-04-06T13:17:04Z
dc.date.issued	2015
dc.description.abstract	The growth and maintenance of bacteria on agar plates (Petri dishes) has long been a common practice in microbiology. The number of colonies in a culture is usually counted manually to calculate the concentration of bacteria, however, this process is time-consuming, tedious and error prone. Most automated counting systems, existing on the literature, perform adequately when the colonies are well spaced, large, circular in shape and with good contrast from the background. When these assumptions are violated, most automated colony analysis systems can rapidly lose reliability, accuracy and utility. To address the above problems, the goal of this study is to design and implement a cost-effective, software-centred system that accepts general digital camera images as its input, for detecting as well as enumerating bacterial colonies in a fully automatic manner. An interactive semi-automatic system is also proposed to overcome any error from fully automatic system. The two systems (automated and interactive) combine thresholding, median filter and morphological operations to segment the colonies on a Petri dish image. The next step consists in separating the individual colonies from the clustered colonies. Then, the units colonies are counted. To separate and count the clustered colonies, the automatic system uses a watershed transformation and the interactive system uses the clicks of the user. The proposed systems are capable to reduce the manpower and time required for counting colonies while producing correct colony counting.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Ribeiro, J.E.; Martins, Ramiro; Monteiro, Fernando C. (2015). Interactive/automated method to count bacterial colonies. In III Encontro de Jovens Investigadores do IPB. Bragança, Portugal	pt_PT
dc.identifier.uri	http://hdl.handle.net/10198/16731
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Instituto Politécnico de Bragança	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Colony counter	pt_PT
dc.subject	Colony forming unit	pt_PT
dc.subject	Colony segmentation	pt_PT
dc.subject	Interactive methods	pt_PT
dc.title	Interactive/automated method to count bacterial colonies	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Bragança, Portugal	pt_PT
oaire.citation.endPage	55	pt_PT
oaire.citation.startPage	55	pt_PT
oaire.citation.title	III Encontro de Jovens Investigadores do IPB	pt_PT
person.familyName	Ribeiro
person.familyName	Martins
person.familyName	Monteiro
person.givenName	J.E.
person.givenName	Ramiro
person.givenName	Fernando C.
person.identifier	R-000-6Y8
person.identifier.ciencia-id	0F15-FB62-29DB
person.identifier.ciencia-id	EA17-F39C-29C7
person.identifier.ciencia-id	2019-BDBF-10E2
person.identifier.orcid	0000-0001-6300-148X
person.identifier.orcid	0000-0003-4327-7782
person.identifier.orcid	0000-0002-1421-8006
person.identifier.rid	G-3839-2018
person.identifier.rid	H-9213-2016
person.identifier.scopus-author-id	25638652400
person.identifier.scopus-author-id	8986162600
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT
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