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Dilution and magnification effects on image analysis applications in activated sludge characterization

2010Journal article Open access
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dc.contributor.authorMesquita, D.P.
dc.contributor.authorDias, O.
dc.contributor.authorElias, R.A.V.
dc.contributor.authorAmaral, António L.
dc.contributor.authorFerreira, E.C.
dc.date.accessioned2018-06-08T14:39:10Z
dc.date.available2018-06-08T14:39:10Z
dc.date.issued2010
dc.description.abstractThe properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.pt_PT
dc.description.sponsorshipThe authors acknowledge financial support to D.P.M. and O.D. through the grant SFRH/BD/32329/2006 and the project POCI/AMB/57069/2004, respectively, provided by Fundação para a Ciência e Tecnologia ~Portugal!. The authors also express their gratitude to AGERE ~Empresa de Águas, Efluentes e Resíduos de Braga – EM! and AGS ~Administração e Gestão de Sistemas de Salubridade, S.A.! for their cooperation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMesquita, D.P.; Dias, O.; Elias, R.A.V.; Amaral, A.L.; Ferreira, E.C. (2010). Dilution and magnification effects on image analysis applications in activated sludge characterization. Microscopy and Microanalysis. ISSN 1431-9276. 16:5, p. 561-568pt_PT
dc.identifier.doi10.1017/S1431927610093785pt_PT
dc.identifier.issn1431-9276
dc.identifier.urihttp://hdl.handle.net/10198/17685
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectActivated sludgept_PT
dc.subjectImage analysispt_PT
dc.subjectDilutionpt_PT
dc.subjectMagnificationpt_PT
dc.subjectAggregatespt_PT
dc.subjectFilamentspt_PT
dc.titleDilution and magnification effects on image analysis applications in activated sludge characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F32329%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FAMB%2F57069%2F2004/PT
oaire.citation.endPage568pt_PT
oaire.citation.issue05pt_PT
oaire.citation.startPage561pt_PT
oaire.citation.titleMicroscopy and Microanalysispt_PT
oaire.citation.volume16pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamPOCI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication24b86646-ab42-44ac-a866-7f68464e153f
relation.isProjectOfPublication9d6bbb8e-69f2-4f21-947e-19af5bf920e7
relation.isProjectOfPublication.latestForDiscovery24b86646-ab42-44ac-a866-7f68464e153f

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