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Frequent parasitism of Apis mellifera by Trypanosomatids in geographically isolated areas with restricted beekeeping movements

2023Journal article Open access
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dc.contributor.authorAguado-López, Daniel
dc.contributor.authorBartolomé, Carolina
dc.contributor.authorLopes, Ana
dc.contributor.authorHenriques, Dora
dc.contributor.authorSegura, Sara K.
dc.contributor.authorMaside, Xulio
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHiges, Mariano
dc.contributor.authorMartín-Hernández, Raquel
dc.date.accessioned2024-02-16T14:36:32Z
dc.date.available2024-02-16T14:36:32Z
dc.date.issued2023
dc.description.abstractTrypanosomatids form a group of high prevalence protozoa that parasitise honey bees, with Lotmaria passim as the predominant species worldwide. However, the knowledge about the ecology of trypanosomatids in isolated areas is limited. The Portuguese archipelagos of Madeira and Azores provide an interesting setting to investigate these parasites because of their geographic isolation, and because they harbour honey bee populations devoid of two major enemies: Varroa destructor and Nosema ceranae. Hence, a total of 661 honey bee colonies from Madeira and the Azores were analysed using different molecular techniques, through which we found a high prevalence of trypanosomatids despite the isolation of these islands. L. passim was the predominant species and, in most colonies, was the only one found, even on islands free of V. destructor and/or N. ceranae with severe restrictions on colony movements to prevent the spread of them. However, islands with V. destructor had a significantly higher prevalence of L. passim and, conversely, islands with N. ceranae did not shown any significant correlation with the trypanosomatid. Crithidia bombi was detected in Madeira and on three islands of the Azores, almost always coincident with L. passim. By contrast, Crithidia mellificae was not detected in any sample. A high-throughput sequencing analysis distinguished two main haplotypes of L. passim, which accounted for 98% of the total sequence reads. This work suggests that L. passim and C. bombi are parasites that have been associated with honey bees predating the spread of V. destructor and N. ceranae.pt_PT
dc.description.sponsorshipThis work was funded by the Consejería de Educación, Cultura y Deportes, of the Junta de Castilla – La Mancha (European Regional Development Fund) Project No. SBPLY/19/180501/000334, and through the program COMPETE 2020—POCI (Programa Operacional para a Competividade e Internacionalização) and FCT (Fundação para a Ciência e a Tecnologia) in the framework of the project BeeHappy (POCI-01–0145-FEDER-029871). INCRECYT program was funded by ESF/EC (European Social Funds). D.A-L contract was funded by the Ministerio de Asuntos Económicos y Transformación Digital (Grant No. PRE2018-084878, RTA2017-00004-C02-01). A.R.L was funded by Fundação para a Ciência e Tecnologia (FCT) through the individual research grant SFRH/BD/143627/2019. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAguado-López, Daniel; Bartolomé, Carolina; Lopes, Ana Rita; Henriques, Dora; Segura, Sara K.; Maside, Xulio; Pinto, M. Alice; Higes, Mariano; Martín-Hernández, Raquel (2023). Frequent parasitism of Apis mellifera by Trypanosomatids in geographically isolated areas with restricted beekeeping movements. Microbial Ecology. ISSN 0095-3628. 86:4, p. 2655-2665pt_PT
dc.identifier.doi10.1007/s00248-023-02266-ypt_PT
dc.identifier.eissn1432-184X
dc.identifier.issn0095-3628
dc.identifier.urihttp://hdl.handle.net/10198/29503
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoney beept_PT
dc.subjectTrypanosomatidspt_PT
dc.subjectLotmaria passimpt_PT
dc.subjectPCRpt_PT
dc.subjectSequencingpt_PT
dc.subjectVarroa destructorpt_PT
dc.titleFrequent parasitism of Apis mellifera by Trypanosomatids in geographically isolated areas with restricted beekeeping movementspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143627%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.endPage2665pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage2655pt_PT
oaire.citation.titleMicrobial Ecologypt_PT
oaire.citation.volume86pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLopes
person.familyNameHenriques
person.familyNamePinto
person.givenNameAna
person.givenNameDora
person.givenNameM. Alice
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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