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Polyphenolic composition and bioactive properties of Cynara cardunculus var. altilis petioles: influence of the harvesting time

2021Conference object Open access
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dc.contributor.authorMandim, Filipa
dc.contributor.authorPetropoulos, Spyridon Α.
dc.contributor.authorDias, Maria Inês
dc.contributor.authorPinela, José
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-01-20T15:29:01Z
dc.date.available2022-01-20T15:29:01Z
dc.date.issued2021
dc.description.abstractCardoon (Cynara cardunculus L.) belongs to the Asteraceae family and is native to the Mediterranean basin. It comprises three varieties: the wild cardoon or var. sylvestris, the domesticated cardoon (i.e., var. altilis DC.), and the globe artichoke (i.e., var. Scolymus). 1 This species is highly consumed for culinary and medicinal purposes in the Mediterranean countries, partly due to its attributed properties (e.g., choleretic, diuretic, hypocholesterolemic, hepatoprotective). It also has several industrial applications in different sectors (e.g., cheese manufacturing, pharmacy, production of bioenergy and biomass), which may contribute significantly to the economic valorisation of the species. Several studies refer to the influence that parameters such as the plant tissue, genetic information, and maturation stage may have on the phenolic compounds composition and, therefore, on its biological activities,1,2 so that its most efficient use is gaining attention. For this reason, the present work aims to valorise underused parts of cardoon and, simultaneously, evaluate the effect of harvesting time on the phenolic composition and biological potential of cardoon petioles. Cardoon petioles were collected in central Greece throughout the full growth cycle at sixteen harvest times (samples P1 – plant regrowth to P16 – plant senescence). The polyphenolic composition was analysed by HPLC-DAD-ESI/MS. The antioxidant activity was evaluated using two cell-based assays: thiobarbituric acid reactive substances (TBARS) formation inhibition and oxidative haemolysis inhibition (OxHLIA). The anti-inflammatory activity was determined through the extracts capacity to inhibit the pro-inflammatory mediator nitric oxide (NO) using a murine macrophage cell line (RAW 246.7). The cytotoxic potential was evaluated against four tumour cell lines (breast carcinoma – MCF-7; non-small cell lung cancer – NCI-H460; hepatocellular carcinoma – HepG2; cervical carcinoma – HeLa), and the hepatotoxicity was determined against a non-tumour cell line (PLP2) by the sulforhodamine B assay. Fifteen phenolic compounds (10 phenolic acids and 5 flavone/ol glycosides) were tentatively identified. 5-O-Caffeoylquinic and cis 3,5-O-dicaffeoylquinic acids were the phenolic compounds present in the highest abundance in the studied stages of maturation. Samples at more advanced maturation stages presented the highest content in polyphenols (101 mg/g extract), and the highest capacity to inhibit the formation of TBARS (IC50 = 5.0 μg/mL). On the other hand, samples at intermediate maturation stages exhibited the highest cytotoxic and anti-inflammatory activities. Moreover, samples of immature petioles stood out with the highest effectiveness against oxidative haemolysis (IC50 = 65 and 180 μg/mL at Δt of 60 and 120 min, respectively). In conclusion, our results indicate a significant effect of harvesting time on bioactive properties and chemical composition of cardoon petioles. However, further studies are needed to correlate specific compounds with the biological properties demonstrated by cardoon petioles, as well to define the optimum harvesting time and the application of agronomic practices (e.g., irrigation, fertilization) for obtaining the highest bioactive potential.pt_PT
dc.description.sponsorshipWe would like to thank grateful to the Foundation for Science and Technology (FCT, Portugal) for F. Mandim’s PhD grant (SFRH/BD/146614/2019); L. Barros and M.I. Dias thank FCT, P.I. for their contracts through the institutional scientific employment program-contract, and J. Pinela (CEECIND/01011/2018) through the individual scientific employment program-contract. This work was financially through national funds FCT/MCTES to CIMO (UIDB/00690/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMandim, Filipa; Petropoulos, Spyridon A.; Dias, Maria Inês; Pinela, José; Santos Buelga, Celestino; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Polyphenolic composition and bioactive properties of Cynara cardunculus var. altilis petioles: Influence of the harvesting time. In XV Encontro de Química dos Alimentos. Funchal. ISBN 978-989-8805-68-3pt_PT
dc.identifier.isbn978-989-8805-68-3
dc.identifier.urihttp://hdl.handle.net/10198/24804
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCEECIND/01011/2018pt_PT
dc.relationIntegrated strategies for the development of a novel functional beverage with sustainable production systems
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCynara cardunculuspt_PT
dc.subjectBioactive propertiespt_PT
dc.titlePolyphenolic composition and bioactive properties of Cynara cardunculus var. altilis petioles: influence of the harvesting timept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleIntegrated strategies for the development of a novel functional beverage with sustainable production systems
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F146614%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceFunchal, Portugalpt_PT
oaire.citation.titleXV Encontro de Química dos Alimentospt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMandim
person.familyNameDias
person.familyNamePinela
person.familyNameFerreira
person.familyNameBarros
person.givenNameFilipa
person.givenNameMaria Inês
person.givenNameJosé
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifier144781
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person.identifier.orcid0000-0002-6494-2326
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person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridB-4466-2014
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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