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Beneficial effect of bee venom and its major components on facial nerve injury induced in mice

2023Journal article Open access
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dc.contributor.authorEr-Rouassi, Hafsa
dc.contributor.authorBakour, Meryem
dc.contributor.authorTouzani, Soumaya
dc.contributor.authorVilas-Boas, Miguel
dc.contributor.authorFalcão, Soraia
dc.contributor.authorVidal, Catherine
dc.contributor.authorLyoussi, Badiâa
dc.date.accessioned2014-10-29T12:05:00Z
dc.date.available2014-10-29T12:05:00Z
dc.date.issued2023
dc.description.abstractPeripheral nerve injury (PNI) is a health problem that affects many people worldwide. This study is the first to evaluate the potential effect of bee venom (BV) and its major components in a model of PNI in the mouse. For that, the BV used in this study was analyzed using UHPLC. All animals underwent a distal section-suture of facial nerve branches, and they were randomly divided into five groups. Group 1: injured facial nerve branches without any treatment. Group 2: the facial nerve branches were injured, and the normal saline was injected similarly as in the BV-treated group. Group 3: injured facial nerve branches with local injections of BV solution. Group 4: injured facial nerve branches with local injections of a mixture of PLA2 and melittin. Group 5: injured facial nerve branches with local injection of betamethasone. The treatment was performed three times a week for 4 weeks. The animals were submitted to functional analysis (observation of whisker movement and quantification of nasal deviation). The vibrissae muscle re-innervation was evaluated by retrograde labeling of facial motoneurons in all experimental groups. UHPLC data showed 76.90 +/- 0.13%, 11.73 +/- 0.13%, and 2.01 +/- 0.01%, respectively, for melittin, phospholipase A2, and apamin in the studied BV sample. The obtained results showed that BV treatment was more potent than the mixture of PLA2 and melittin or betamethasone in behavioral recovery. The whisker movement occurred faster in BV-treated mice than in the other groups, with a complete disappearance of nasal deviation two weeks after surgery. Morphologically, a normal fluorogold labeling of the facial motoneurons was restored 4 weeks after surgery in the BV-treated group, but no such restoration was ever observed in other groups. Our findings indicate the potential of the use of BV injections to enhance appropriate functional and neuronal outcomes after PNI.por
dc.identifier.citationEr-Rouassi, Hafsa; Bakour, Meryem; Touzani, Soumaya; Vilas-Boas, Miguel; Falcão, Soraia; Vidal, Catherine; Lyoussi, Badiâa (2023). Beneficial effect of bee venom and its major components on facial nerve injury induced in mice. Biomolecules. eISSN 2218-273X. 13:4, p. 1-17por
dc.identifier.doi10.3390/biom13040680
dc.identifier.eissn2218-273X
dc.identifier.urihttp://hdl.handle.net/10198/11169
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherMDPI
dc.subjectPeripheral nerve injury
dc.subjectBee venom
dc.subjectMelittin
dc.subjectPLA2
dc.subjectFacial nerve branches recovery
dc.subjectSwiss mice
dc.titleBeneficial effect of bee venom and its major components on facial nerve injury induced in micepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiomoleculespor
person.familyNameVilas-Boas
person.familyNameFalcão
person.givenNameMiguel
person.givenNameSoraia I.
person.identifier1693134
person.identifier711432
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.orcid0000-0002-8665-5280
person.identifier.orcid0000-0003-3735-6951
person.identifier.ridI-5949-2013
person.identifier.scopus-author-id6602648497
person.identifier.scopus-author-id14832459200
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf58280c8-298b-4e4c-9f7c-4517300d3382
relation.isAuthorOfPublication5746ab3e-8ffe-4e26-9a41-8392cf031f28
relation.isAuthorOfPublication.latestForDiscoveryf58280c8-298b-4e4c-9f7c-4517300d3382

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