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Escherichia coli phylogenetic and antimicrobial pattern as an indicator of anthropogenic impact on threatened freshwater mussels

2023Journal article Open access
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dc.contributor.authorVarandas, Simone
dc.contributor.authorFernandes, Conceição
dc.contributor.authorCabecinha, Edna
dc.contributor.authorGomes, Sónia
dc.contributor.authorSilva, Gabriela Jorge da
dc.contributor.authorSaavedra, Maria José
dc.date.accessioned2023-12-19T10:03:39Z
dc.date.available2023-12-19T10:03:39Z
dc.date.issued2023
dc.description.abstractFreshwater bivalves are widely used as accumulation indicators and monitoring tools for assessing contaminant effects on different levels of biological integration. This pilot study aimed to explore the phylogenetic diversity of Escherichia coli isolated from freshwater mussels (Margaritifera margaritifera and Potomida littoralis) and characterize their phenotypes and antibiotic resistance profiles. Samples were collected in the Rabaçal and Tua Rivers, in the Douro basin, Portugal—two sites representing different levels of anthropogenic contamination. Antimicrobial susceptibility testing was performed via the disk diffusion method with 21 antibiotics. Results showed that 31% of isolates were multidrug-resistant (MDR). Thus, freshwater mussels provide an effective and time-integrated approach for identifying/quantifying fecal indicators, including MDR bacteria. PCR-based assays were designed for assessing phylogenetic E. coli groups. Among the E. coli isolates, the highest prevalence (44%) was observed in group D or E, followed by group E or Clade I (25%), group A (19%), and group B1 (13%). E. coli isolated from M. margaritifera predominantly exhibited a higher prevalence of phylogroups D or E, whereas E. coli from P. littoralis showed associations with phylogroups E or clade I, B1, A, and D or E. Our results provide new insights into the phylogenetic diversity of E. coli in freshwater bivalves. Additionally, the findings highlight the possible linkage of phylogroups with the host species, the geographical location in the water stream, and human activity. Using E. coli as a bioindicator isolated from freshwater mussels helps us grasp how human activities affect the environment. This study has important implications for those interested in safeguarding water resources, especially in tackling antibiotic resistance in aquatic ecosystems.pt_PT
dc.description.sponsorshipThis research was supported by the Portuguese Foundation for Science and Technology (FCT) and the Operational Competitiveness Programme (COMPETE), under the projects UIDB/AGR/ 04033/2020 (CITAB) and Inov4Agro; UIDB/CVT/00772/2020 (CEVAV) and AL4AnimalS; UIDB/ 00690/2020 (CIMO-IPB), and UIDB/NEU/04539/2020 (CNC).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVarandas, Simone; Fernandes, Conceição; Cabecinha, Edna; Gomes, Sónia; Silva, Gabriela Jorge da; Saavedra, Maria José (2023). Escherichia coli phylogenetic and antimicrobial pattern as an indicator of anthropogenic impact on threatened freshwater mussels. ISSN 2079-6382. Antibiotics. 12:9, p. 1-10pt_PT
dc.identifier.doi10.3390/antibiotics12091401pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10198/28973
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationVeterinary and Animal Science Research Centre
dc.relationMountain Research Center
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEscherichia colipt_PT
dc.subjectOne Health-EcoHealthpt_PT
dc.subjectAntimicrobial resistancept_PT
dc.subjectPotomida littoralispt_PT
dc.subjectMargaritifera margaritíferapt_PT
dc.subjectEcological integritypt_PT
dc.titleEscherichia coli phylogenetic and antimicrobial pattern as an indicator of anthropogenic impact on threatened freshwater musselspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleMountain Research Center
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.citation.endPage10pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFernandes
person.givenNameConceição
person.identifier15483
person.identifier.ciencia-id6514-3D41-4534
person.identifier.orcid0000-0003-2873-501X
person.identifier.scopus-author-id23018336600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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