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In vitro blood flow and cell-free layer in hyperbolic microchannels: visualizations and measurments

2016Journal article Open access
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dc.contributor.authorRodrigues, Raquel Oliveira
dc.contributor.authorLopes, Raquel
dc.contributor.authorPinho, Diana
dc.contributor.authorPereira, Ana I.
dc.contributor.authorGarcia, Valdemar
dc.contributor.authorGassmann, Stefan
dc.contributor.authorSousa, Patrícia C.
dc.contributor.authorLima, Rui A.
dc.date.accessioned2016-03-31T09:54:10Z
dc.date.available2016-03-31T09:54:10Z
dc.date.issued2016
dc.description.abstractRed blood cells (RBCs) in microchannels has tendency to undergo axial migration due to the parabolic velocity profile, which results in a high shear stress around wall that forces the RBC to move towards the centre induced by the tank treading motion of the RBC membrane. As a result there is a formation of a cell free layer (CFL) with extremely low concentration of cells. Based on this phenomenon, several works have proposed microfluidic designs to separate the suspending physiological fluid from whole in vitro blood. This study aims to characterize the CFL in hyperbolic-shaped microchannels to separate RBCs from plasma. For this purpose, we have investigated the effect of hyperbolic contractions on the CFL by using not only different Hencky strains but also varying the series of contractions. The results show that the hyperbolic contractions with a Hencky strain of 3 and higher, substantially increase the CFL downstream of the contraction region in contrast with the microchannels with a Hencky strain of 2, where the effect is insignificant. Although, the highest CFL thickness occur at microchannels with a Hencky strain of 3.6 and 4.2 the experiments have also shown that cells blockage are more likely to occur at this kind of microchannels. Hence, the most appropriate hyperbolic-shaped microchannels to separate RBCs from plasma is the one with a Hencky strain of 3.pt_PT
dc.description.sponsorshipThe authors acknowledge the financial support provided by PTDC/SAU-ENB/116929/2010 and EXPL/EMS-SIS/2215/2013 from FCT (Fundação para a Ciência e a Tecnologia), COMPETE, QREN and European Union (FEDER). R.O. Rodrigues, D. Pinho and P. C. Sousa acknowledge the scholarships SFRH/BD/97658/2013, SFRH/BD/89077/2012 and SFRH/BPD/75258/2010, respectively, all attributed by FCT.
dc.identifier.citationRodrigues, Raquel O.; Lopes, Raquel; Pinho, Diana; Pereira, Ana I.; Garcia, Valdemar; Gassmann, Stephan; Sousa, Patrícia C.; Lima, R. (2016). In vitro blood flow and cell-free layer in hyperbolic microchannels: visualizations and measurments. BioChip Journal. ISSN 1976-0280. 7:1, p. 1-9pt_PT
dc.identifier.doi10.1007/s13206-016-0102-2pt_PT
dc.identifier.issn1976-0280
dc.identifier.urihttp://hdl.handle.net/10198/12856
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
dc.relationMAGNETIC CARBON NANOSTRUCTURES AND STUDY OF THEIR TRANSPORT IN MICROFLUIDIC DEVICES FOR HYPERTHERMIA
dc.relationBLOOD RHEOLOGY AND DEVELOPMENT OF BLOOD ANALOGUES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBloodpt_PT
dc.subjectCell-free layerpt_PT
dc.subjectHyperbolic microchanelspt_PT
dc.subjectHencky strainpt_PT
dc.subjectMicrocirculationpt_PT
dc.subjectMicrofluid systemspt_PT
dc.titleIn vitro blood flow and cell-free layer in hyperbolic microchannels: visualizations and measurmentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability
oaire.awardTitleMAGNETIC CARBON NANOSTRUCTURES AND STUDY OF THEIR TRANSPORT IN MICROFLUIDIC DEVICES FOR HYPERTHERMIA
oaire.awardTitleBLOOD RHEOLOGY AND DEVELOPMENT OF BLOOD ANALOGUES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F116929%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FEMS-SIS%2F2215%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97658%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F75258%2F2010/PT
oaire.citation.endPage9pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBioChip Journalpt_PT
oaire.citation.volume7pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamCOMPETE
oaire.fundingStreamSFRH
person.familyNamePinho
person.familyNamePereira
person.familyNameGarcia
person.familyNameLima
person.givenNameDiana
person.givenNameAna I.
person.givenNameValdemar
person.givenNameRui A.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-id0716-B7C2-93E4
person.identifier.ciencia-idEE12-C3FB-349D
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0003-3803-2043
person.identifier.orcid0000-0002-3941-2438
person.identifier.orcid0000-0003-3428-637X
person.identifier.ridF-3168-2010
person.identifier.ridH-5157-2016
person.identifier.scopus-author-id15071961600
person.identifier.scopus-author-id18437397800
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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