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Enzymes and secondary metabolites profiles of Gnomoniopsis smithogilvyi are affected by chestnut medium

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dc.contributor.authorSampaio, Ana
dc.contributor.authorCamelo, Verónica
dc.contributor.authorFraga, Irene
dc.contributor.authorGomes, A.
dc.contributor.authorSulyok, Michael
dc.contributor.authorGomes-Laranjo, José
dc.contributor.authorDias, Albino A.
dc.contributor.authorRodrigues, Paula
dc.date.accessioned2023-02-24T15:22:04Z
dc.date.available2023-02-24T15:22:04Z
dc.date.issued2021
dc.description.abstractChestnut tree (Castanea sativa Mill) is a crop with high economic and social importance in Trás-os-Montes Region. The pre and post-harvest quality of its nuts can be affected by many factors, being the lasses dueto fungi very large. During the last decade a new endophyte fungus, Gnomoniopsis smithogilvyi, causing brown rot, has been isolated from both rotten and healthy fruits, and its growth seems to be related with the increase of air temperatura and rainfall during spring. The present study aims to understand the influence of chestnut substrate on G. smithogilvyi biochemistry, in arder to develop strategies to reduce its incidence in the fruit and improve the contrai of brown rot. lnitially, the presence of the enzymes involved in the decomposition of chestnuts, such as amylase, proteases, carboxymethylcellulase (CMCase), ~-glucosidase, xylanase, among others, was investigated. Further, some hydrolase activities were evaluated in two isolates of G. smithogilvyi incubated in potato (PDB) and chestnut media (CM) along an incubation period (3, 7 and 14 days), in arder to study the effects "isolate", "medium" and "incubation period" on enzymes production. Also, the synthesis of secondary metabolites in fungai dry biomass was assessed by Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Ali isolates produced the enzymes screened and, in general, the enzyme production varied between the isolates (P< 0.0000), the media (P< 0.0000) and along the incubation period (P< 0.0000). Amylase activity was positively correlated with xylanase (P< 0.0001), CMCase (P< 0.0001), and Avicelase {P< 0.01), xylanase with CMCase (P< 0.01) and ~-glucosidase with Avicelase (P< 0.0001 ). By contrast, ~-glucosidase was negatively correlated with CMCase {P< 0.01 ). Twenty secondary metabolites were detected along the incubation: eight in both isolates and media, tive and three only in isolates grew in PD o r CM, respectively. Some of these metabolites had been associated with other fungi and have known biological activities.pt_PT
dc.description.sponsorshipThis work was suported by "ValorCast- Valorização da castanha e otimização da sua comercialização" (PDR2020-1.0.1- FEADER-032034}, funded by Fundo Europeu Agrfcola de Desenvolvimento Rural (FEADER} and the Portuguesa Government (Ação 1.1 Grupos Operacionais, Medida 1. Inovação, PDR 2020- Programa de Desenvolvimento Rural do Continente). AS, IF, JGL, AAD, are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CITAB (UIDB/04033/2020) and PR to CIMO (UIDB/00690/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSampaio, Ana; Camelo, Verónica; Fraga, I.; Gomes, Ana; Sulyok, Michael; Gomes-Laranjo, José; Dias, Albino A.; Rodrigues, Paula (2021). Enzymes and secondary metabolites profiles of Gnomoniopsis smithogilvyi are affected by chestnut médium. In MicroBiotec21: book of abstracts. Lisboa p. 569-570pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/27199
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectChestnut treept_PT
dc.subjectCastanea sativa Mill.pt_PT
dc.titleEnzymes and secondary metabolites profiles of Gnomoniopsis smithogilvyi are affected by chestnut mediumpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.titleMicroBiotec21: book of abstractspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.givenNamePaula
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.orcid0000-0002-3789-2730
person.identifier.scopus-author-id7102639646
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb864b8c1-d66a-419c-b1ac-e6193f93eca6
relation.isAuthorOfPublication.latestForDiscoveryb864b8c1-d66a-419c-b1ac-e6193f93eca6
relation.isProjectOfPublicationac4fb709-719a-450b-8c96-17592d46f5e9
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

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