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Use of iRNA in the post-transcriptional gene silencing of necrosis-inducing Phytophthora protein 1(NPP1) in Phytophthora cinnamomi

2023Journal article Open access
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dc.contributor.authorPascoal‑Ferreira, Patrick
dc.contributor.authorChahed, Abdessalem
dc.contributor.authorCosta, Rodrigo Arthur Fonseca
dc.contributor.authorBranco, Iuliia
dc.contributor.authorChoupina, Altino
dc.date.accessioned2023-09-01T14:24:00Z
dc.date.available2023-09-01T14:24:00Z
dc.date.issued2023
dc.description.abstractPhytophthora cinnamomi is an Oomycetes associated with soil, this Oomycete is one of the most destructive species of Phytophthora, being responsible for the decline of more than 5000 ornamental, forest, or fruit plants. It can secrete a class of protein NPP1 (Phytophthora necrosis inducing protein 1), responsible for inducing necrosis in leaves and roots of plants, leading to their death. Objective This work will report the characterization of the Phytophthora cinnamomi NPP1 gene responsible for the infec tion of Castanea sativa roots and will characterize the mechanisms of interaction between Phytophthora cinnamomi and Castanea sativa, by gene silencing NPP1 from Phytophthora cinnamomi mediated by RNAi. Methods and results For silencing a part of the coding region of the NPP1 gene, was placed in the sense and antisense directions between an intron and ligated to the integrative vector pTH210. Cassette integration was confrmed by PCR and sequencing on the hygromycin-resistant Phytophthora cinnamomi transformants. Transformants obtained with the silenced gene was used to infect Castanea sativa. Conclusions Plants infected with these transformants showed a great reduction in disease symptoms, confrming iRNA as a potential alternative biological tool in the study of molecular factors, and in the control and management of Phytophthora cinnamomi.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPatrick Pascoal-Ferreira; Abdessalem Chahed; Rodrigo Costa; Iuliia Branco; Altino Choupina (2023). Use of iRNA in the post-transcriptional gene silencing of necrosis-inducing Phytophthora protein 1(NPP1) in Phytophthora cinnamomi. Molecular Biology Reports. ISSN 0301-4851. 50:8, p. 6493–6504pt_PT
dc.identifier.doi10.1007/s11033-023-08562-7pt_PT
dc.identifier.eissn1573-4978
dc.identifier.issn0301-4851
dc.identifier.urihttp://hdl.handle.net/10198/28702
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPhytophthta cinnamomipt_PT
dc.subjectCastanea sativapt_PT
dc.subjectiRNApt_PT
dc.subjectHairpin-RNApt_PT
dc.subjectNPP1pt_PT
dc.titleUse of iRNA in the post-transcriptional gene silencing of necrosis-inducing Phytophthora protein 1(NPP1) in Phytophthora cinnamomipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.endPage12pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMolecular Biology Reportspt_PT
oaire.citation.volume50pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameChoupina
person.givenNameAltino
person.identifier587972
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication.latestForDiscovery314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isProjectOfPublicationd6683ba1-d253-48e4-968e-35106ce7b750
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