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Assessment of physiological conditions in e. Coli fermentations by epifluorescent microscopy and image analysis

2009Journal article Restricted access
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dc.contributor.authorCarneiro, Sónia
dc.contributor.authorAmaral, António L.
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorDias, Teresa
dc.contributor.authorPeres, António M.
dc.contributor.authorFerreira, Eugénio C.
dc.contributor.authorRocha, Isabel
dc.date.accessioned2011-02-01T15:26:34Z
dc.date.available2011-02-01T15:26:34Z
dc.date.issued2009
dc.description.abstractThe development of monitoring methods for assessing the physiological state of microorganisms during recombinant fermentation processes has been encouraged by the need to evaluate the influence of processing conditions in recombinant protein production. In this work, a technique based on microscopy and image analysis was developed that allows the simultaneous quantification of parameters associated with viability and fluorescent protein production in recombinant Escherichia coli fermentations. Images obtained from light microscopy with phase contrast are used to assess the total number of cells in a given sample and, from epifluorescence microscopy, both protein producing and injured cells are evaluated using two different fluorochromes: propidium iodide and enhanced yellow fluorescent protein. This technique revealed the existence of different cell populations in the recombinant E. coli fermentation broth that were evaluated along four batch fermentations, complementing information obtained with standard techniques to study the effects of the temperature and induction time in recombinant protein production processes.por
dc.description.sponsorshiprecSysBio project (reference FCT POCI/BIO/60139/2004por
dc.identifier.citationCarneiro, Sónia; Amaral, António L.; Veloso, Ana C.A.; Dias, Teresa; Peres, António M.; Ferreira, Eugénio C.; Rocha, Isabel (2009). Assessment of physiological conditions in e. Coli fermentations by epifluorescent microscopy and image analysis. Biotechnology Progress. ISSN 8756-7938. 25:3, p. 882-891por
dc.identifier.doi10.1002/btpr.134
dc.identifier.issn8756-7938
dc.identifier.urihttp://hdl.handle.net/10198/3231
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectRecombinant E. coli fermentationspor
dc.subjectEpifluorescent microscopypor
dc.subjectPhysiology assessmentpor
dc.subjectImage analysispor
dc.titleAssessment of physiological conditions in e. Coli fermentations by epifluorescent microscopy and image analysispor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage891por
oaire.citation.issue25por
oaire.citation.startPage882por
oaire.citation.titleBiotechnology Progresspor
person.familyNameDias
person.familyNamePeres
person.givenNameTeresa
person.givenNameAntónio
person.identifier142703
person.identifier107333
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id7102331969
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication87c9cae0-314d-4622-ba85-4d3ec5726606
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery87c9cae0-314d-4622-ba85-4d3ec5726606

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