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Artificial insemination with two different vaginoscopes of Churra Galega Bragançana ewes

dc.contributor.authorQuintas, Helder
dc.contributor.authorSilva, Luis
dc.contributor.authorMateus, Óscar
dc.contributor.authorRamos, Juan Jose
dc.contributor.authorFerrer, Luis Miguel
dc.contributor.authorCorreia, Teresa Montenegro
dc.contributor.authorLacasta, Delia
dc.contributor.authorValentim, Ramiro
dc.date.accessioned2023-05-09T13:51:04Z
dc.date.available2023-05-09T13:51:04Z
dc.date.issued2021
dc.description.abstractThe main goal of this paper was to compare the efficiency of two speculums in the AI of Portuguese Churra Galega Bragançana (CGB) ewes. This study was performed at Braganza (latitude 41° 48’ 33’’N, longitude 6° 44’ 3’’W, and Altitude 670 meters) between April 1st and June 5th. Forty-nine CGB ewes aged between 2 to 8 years were used. Body condition was scored according to the Australian classification table. On April 12th ewes estrous was synchronized by long-term FGA (20 mg) or CIDR (0.35 g) treatments and the injection of 500 UI of eCG at vaginal devices withdrawal. Ovarian responses to treatments were assayed by progesterone plasmatic levels. Blood samples were collected for 5 days post-eCG administration. Ewes were artificially inseminated 53 + 1 hours after eCG injection with chilled semen using an IMV speculum (n = 23) or a new speculum developed by Ovígén (n = 26). Ejaculates were collected by artificial vaginas. Semen analysis was performed using a computer-assisted sperm analysis (CASA) system. Selected ejaculates presented a volume ≥ 2.0 ml, a sperm concentration ≥ 3.0 x 109 cells/ml, motility ≥ 75%, and a percentage of normal sperm cells ≥ 75%. Insemination doses contained at least 200 x 106 sperm cells. Pregnancy diagnosis was performed by ultrasonography 41 days after AI. All ewes presented progesterone plasmatic levels higher than 0.5 ng/ml for the first five days post-eCG injection. About 81.6% of all ewes were pregnant 41 days after AI. Neither age nor body score condition affected significantly the fertility rate (P>0.05). The synchronization protocol had no statistically significant on the fertility rate (FGA + eCG: 84.6% vs. CIDR+ eCG: 78.3%; c2=1.6; P>0.05). The type of speculum had also not statistically significant on fertility rate (IMV: 87.0%vs. Ovígén: 76.9%; c2=3.4; P>0.05). both speculums result in high fertility rates. However, the Ovígén speculum is easier to use since it allows to pin the vaginal opening of the cervix.pt_PT
dc.description.sponsorshipProjecto POCTEP 2014-2020 (0687_OVISPID_2_E)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationQuintas, Helder; Silva, Luis; Mateus, Óscar; Ramos, Juan Jose; Ferrer, Luis Miguel; Correia, Teresa Montenegro; Lacasta, Delia; Valentim, Ramiro (2021). Artificial insemination with two different vaginoscopes of Churra Galega Bragançana ewes. In Book of Abstracts of ISVA2021. Onlinept_PT
dc.identifier.isbn978-84-09-38781-6
dc.identifier.urihttp://hdl.handle.net/10198/28249
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherISVA2021pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArtificial inseminationpt_PT
dc.subjectSpeculumpt_PT
dc.subjectChurra Galega Bragançanapt_PT
dc.subjectEwespt_PT
dc.titleArtificial insemination with two different vaginoscopes of Churra Galega Bragançana ewespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceOnlinept_PT
oaire.citation.endPage61pt_PT
oaire.citation.startPage61pt_PT
oaire.citation.titleBook of Abstracts of ISVA2021pt_PT
oaire.citation.volumeISVA2021pt_PT
person.familyNameQuintas
person.familyNameCorreia
person.familyNameValentim
person.givenNameHelder
person.givenNameTeresa Montenegro
person.givenNameRamiro
person.identifier1486884
person.identifier.ciencia-id6018-8BBD-80AB
person.identifier.ciencia-id1610-5AFD-75A1
person.identifier.ciencia-idB01B-96FF-D524
person.identifier.orcid0000-0002-6934-1669
person.identifier.orcid0000-0002-9640-8320
person.identifier.orcid0000-0002-3514-988X
person.identifier.ridK-2190-2014
person.identifier.scopus-author-id35796342900
person.identifier.scopus-author-id36607620800
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication068e74bc-656d-4fd1-93ed-f7346baa3b69
relation.isAuthorOfPublicationebf24a1e-8016-47d7-be98-2b39fe4e8caa
relation.isAuthorOfPublication7c1f54bd-81e6-45c4-ac4d-6a297ecd0f35
relation.isAuthorOfPublication.latestForDiscovery068e74bc-656d-4fd1-93ed-f7346baa3b69

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