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The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract

2023Journal article Open access
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dc.contributor.authorCorreia, Edmilson Emanuel Monteiro
dc.contributor.authorFigueirinha, Artur
dc.contributor.authorRodrigues, Lisa
dc.contributor.authorPinela, José
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBarros, Lillian
dc.contributor.authorFernandes, Chantal
dc.contributor.authorSalgueiro, Lígia
dc.contributor.authorGonçalves, Teresa
dc.date.accessioned2020-04-28T09:50:48Z
dc.date.available2020-04-28T09:50:48Z
dc.date.issued2023
dc.description.abstractWithania chevalieri, endogenous from Cape Verde, is a medicinal plant used in ethnomedicine with a large spectrum of applications, such as treating skin fungal infections caused by dermatophytes. The aim of this work was to chemically characterize the W. chevalieri crude ethanolic extract (WcCEE), and evaluate its bioactivities as antidermatophytic, antioxidant, anti-inflammatory and anticancer, as well as its cytotoxicity. WcCEE was chemically characterized via HPLC-MS. The minimal inhibitory concentration, minimal fungicidal concentration, time-kill and checkerboard assays were used to study the antidermatophytic activity of WcCEE. As an approach to the mechanism of action, the cell wall components, & beta;-1,3-glucan and chitin, and cell membrane ergosterol were quantified. Transmission electron microscopy (TEM) allowed for the study of the fungal ultrastructure. WcCEE contained phenolic acids, flavonoids and terpenes. It had a concentration-dependent fungicidal activity, not inducing relevant resistance, and was endowed with synergistic effects, especially terbinafine. TEM showed severely damaged fungi; the cell membrane and cell wall components levels had slight modifications. The extract had antioxidant, anti-inflammatory and anti-cancer activities, with low toxicity to non-tumoral cell lines. The results demonstrated the potential of WcCEE as an antidermatophytic agent, with antioxidant, anti-inflammatory and anticancer activity, to be safely used in pharmaceutical and dermocosmetic applications.pt_PT
dc.description.sponsorshipThis work was funded by the European Regional Development Fund (ERDF), through the Centro 2020 Regional Operational Programme: project CENTRO-01-0145-FEDER-000012- HealthyAging2020 and CENTRO-01-0145-FEDER-022095: ViraVector; the COMPETE 2020–Operational Programme for Competitiveness and Internationalisation, and the Portuguese national funds via FCT– Fundação para a Ciência e a Tecnologia, I.P.:UIDB/04539/2020 and UIDP/04539/2020 & Fundação para a Ciência e Tecnologia (FCT)—in the scope of the projects UIDB/04539/2020, UIDP/04539/2020. The authors are also thankful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020); national funding by FCT, P.I., through the institutional scientific employment program contract for R.C.C. and L.B. contracts, and through the individual research grant 2020.06297.
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCorreia, Edmilson Emanuel Monteiro; Figueirinha, Artur; Rodrigues, Lisa; Pinela, José; Calhelha, Ricardo C.; Barros, Lillian; Fernandes, Chantal; Salgueiro, Lígia; Gonçalves, Teresa (2023). The chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extract. Plants. eISSN 2223-7747. 12:13, p. 1-24pt_PT
dc.identifier.doi10.3390/plants12132502pt_PT
dc.identifier.eissn2223-7747
dc.identifier.urihttp://hdl.handle.net/10198/21847
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWithania chevalieript_PT
dc.subjectCape Verdept_PT
dc.subjectAntidermatophytic activitypt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectAnti-inflammatory activity
dc.subjectAnticancer activity
dc.subjectCytotoxicity
dc.titleThe chemical profile, and antidermatophytic, anti-inflammatory, antioxidant and antitumor activities of Withania chevalieri A.E. Gonc. ethanolic extractpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titlePlantspt_PT
person.familyNamePinela
person.familyNameCalhelha
person.familyNameBarros
person.givenNameJosé
person.givenNameRicardo C.
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridB-4466-2014
person.identifier.ridJ-2172-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id35236343600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication706cd9ff-065b-4c98-98a7-5c0a9e45adc4
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relation.isAuthorOfPublication.latestForDiscovery706cd9ff-065b-4c98-98a7-5c0a9e45adc4

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