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Evaluation of the cytotoxicity (HepG2) and chemical composition of polar extracts from the ruderal species Coleostephus myconis (L.) Rchb.f.

2017Journal article Open access
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dc.contributor.authorBessada, Sílvia M.F.
dc.contributor.authorBarreira, João C.M.
dc.contributor.authorSantos, J.V. Araújo
dc.contributor.authorCosta, Carla Sofia
dc.contributor.authorPimentel, Filipa B.
dc.contributor.authorBessa, Maria João
dc.contributor.authorTeixeira, João Paulo Fernandes
dc.contributor.authorOliveira, Beatriz
dc.date.accessioned2018-01-25T10:00:00Z
dc.date.accessioned2018-01-30T11:51:28Z
dc.date.available2018-01-25T10:00:00Z
dc.date.available2018-01-30T11:51:28Z
dc.date.issued2017
dc.description.abstractColeostephus myconis (L.) Rchb.f. (Asteraceae) is a highly disseminated plant species with ruderal and persistent growth. Owing to its advantageous agronomic properties, C. myconis might have industrial applications. However, this species needs to be comprehensively characterized before any potential use. In a previous study, the phenolic composition and antioxidant activity of different C. myconis tissues were characterized. This investigation was extended to examine the cytotoxic potential of selected plant tissues (flowers and green parts) using a HepG2 cell line by utilizing the lysosomal neutral red uptake assay or mitochondrial (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. In addition, the macronutrients content, lipophilic compounds (fatty acids, tocopherols), and amino acids were also determined. C. myconis flowers were used in the senescence stage, which was previously identified as the stage that presented maximal phenolic content and highest antioxidant activity. In contrast, stems and leaves were employed due to their high biomass proportion. Regarding cytotoxicity, mitochondrial and lysosomal damage was only significant when HepG2 cells were exposed to the highest extract concentrations (stems and leaves, 0.9 mg/ml; senescent flowers, 0.3 mg/ml). Chemically, the senescent flowers were mostly characterized by their high levels of fat, amino acids (especially threonine), oleic acid, ß-, and ?-tocopherol, while stems and leaves contained high concentrations of carbohydrates, linolenic acid, and a-tocopherol. In general, these results provide information regarding the threshold concentrations of C. myconis extracts that might be used in different applications without toxicity hazards.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to REQUIMTE (PEst-C/EQB/LA0006/2014) and to CIMO (UID/AGR/00690/2013). J.C.M. Barreira, Carla Costa, and Filipa B. Pimentel thank FCT, POPH-QREN, and FSE for their grants (SFRH/BPD/72802/2010, SFRH/BPD/96196/2013 and SFRH/BD/109042/2015, respectively).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBessada, Sílvia M.F.; Barreira, João C.M.; Santos, J.; Costa, Carla; Pimentel, Filipa B.; Bessa, Maria João; Teixeira, João Paulo; Oliveira, M. Beatriz P.P. (2017). Evaluation of the cytotoxicity (HepG2) and chemical composition of polar extracts from the ruderal species Coleostephus myconis (L.) Rchb.f.. Journal of Toxicology and Environmental Health - Part A: Current Issues. ISSN 1528-7394. 80, p. 641-650en_EN
dc.identifier.doi10.1080/15287394.2017.1286915pt_PT
dc.identifier.issn1528-7394
dc.identifier.urihttp://hdl.handle.net/10198/15258
dc.language.isoeng
dc.peerreviewedyespt_PT
dc.relation2014 - Incentive
dc.relationBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
dc.relationImpact of transplacental exposure to tobacco smoke in the DNA of newborn. Evaluation of genetic damage and epigenetic changes. Evolution throughout childhood.
dc.relationMacroalgae from semi-intensive production: bioactive peptides and their potential applications
dc.titleEvaluation of the cytotoxicity (HepG2) and chemical composition of polar extracts from the ruderal species Coleostephus myconis (L.) Rchb.f.en_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitle2014 - Incentive
oaire.awardTitleBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYS
oaire.awardTitleImpact of transplacental exposure to tobacco smoke in the DNA of newborn. Evaluation of genetic damage and epigenetic changes. Evolution throughout childhood.
oaire.awardTitleMacroalgae from semi-intensive production: bioactive peptides and their potential applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/Incentivo%2FEQB%2FLA0006%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72802%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F96196%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F109042%2F2015/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream5876
oaire.fundingStreamPOR_NORTE
person.familyNameBarreira
person.givenNameJoão C.M.
person.identifier.orcid0000-0003-1233-0990
person.identifier.ridD-8269-2013
person.identifier.scopus-author-id54895546900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticlept_PT
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