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Large Scale Mitochondrial Evidence of C-lineage Dominance in European Honey Bees

datacite.subject.fosCiências Agrárias::Outras Ciências Agrárias
datacite.subject.sdg02:Erradicar a Fome
dc.contributor.authorLi, Fernanda
dc.contributor.authorLopes, Ana
dc.contributor.authorCosta, Maíra
dc.contributor.authorHenriques, Dora
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorYadró García, Carlos A.
dc.contributor.authorAlbo, Alexandre
dc.contributor.authorČereškienė, Laima Blažytė
dc.contributor.authorBrodschneider, Robert
dc.contributor.authorBrusbardis, Valters
dc.contributor.authorCarreck, Norman L.
dc.contributor.authorCharistos, Leonidas
dc.contributor.authorChlebo, Robert
dc.contributor.authorCoffey, Mary F.
dc.contributor.authorDahle, Bjørn
dc.contributor.authorDanneels, Ellen
dc.contributor.authorDobrescu, Constantin
dc.contributor.authorDupleix-Marchal, Anna
dc.contributor.authorFilipi, Janja
dc.contributor.authorGajda, Anna
dc.contributor.authorGratzer, Kristina
dc.contributor.authorGroeneveld, Linn Fenna
dc.contributor.authorHatjina, Fani
dc.contributor.authorJohannesen, Jes
dc.contributor.authorKolasa, Michal
dc.contributor.authorKörmendy-Rácz, János
dc.contributor.authorKovačić, Marin
dc.contributor.authorKristiansen, Preben
dc.contributor.authorMartikkala, Maritta
dc.contributor.authorMcCormack, Grace P.
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorPavlov, Borce
dc.contributor.authorPoirot, Benjamin
dc.contributor.authorPietropaoli, Marco
dc.contributor.authorRadev, Zheko
dc.contributor.authorRaudmets, Aivar
dc.contributor.authorRené-Douarre, Vincent
dc.contributor.authorRoessink, Ivo
dc.contributor.authorŠkerl, Maja Ivana Smodiš
dc.contributor.authorSoland, Gabriele
dc.contributor.authorTitera, Dalibor
dc.contributor.authorSteen, Jozef van der
dc.contributor.authorVarnava, Andri
dc.contributor.authorVejsnæs, Flemming
dc.contributor.authorFedoriak, Mariia M.
dc.contributor.authorZarochentseva, Oksana
dc.contributor.authorWebster, Matthew T.
dc.contributor.authorGraaf, Dirk C.
dc.contributor.authorPinto, M. Alice
dc.date.accessioned2025-07-03T14:56:39Z
dc.date.available2025-07-03T14:56:39Z
dc.date.issued2025
dc.description.abstractIn Europe, distribution of the several endemic honey bee (Apis mellifera) subspecies has suffered a considerable shift in the last century. In particular, beekeepers tend to favour subspecies of Eastern European ancestry (C-lineage), such as the Italian honey bee (A. m. ligustica), due to their perceived docility and high honey production. As a result, large scale migratory beekeeping and trade of C-lineage queens have exposed the native European honey bees to introgression and replacement, jeopardizing their genetic integrity and locally adapted traits. The maternally-inherited and highly polymorphic mitochondrial intergenic region tRNAleu-cox2 is routinely used for the assessment of honey bee diversity and introgression at large geographical scales. In this study, we conducted a survey on tRNAleu-cox2 variation in more than 1300 colonies from 33 European countries to assess current status of mitochondrial diversity patterns in Europe. Total genomic DNA was extracted from the bee thorax followed by PCR amplification of the tRNAleu-cox2 region and sequencing. The haplotypes were identified using alignments in MEGA 11. A clear dominance of C-lineage haplotypes was found (65%). The most prevalent C-lineage haplotype was C2 (54%) followed by C3 (23%) and C1(22%). Contrary to this trend, in Portugal, Spain and Ireland, less than 10% of the colonies exhibit the C-haplotype. Furthermore, the analysed apiaries in isolated protected areas (n=7) revealed a high proportion of colonies of western European (M-lineage) ancestry (80%) with M4 as the most frequent haplotype. African haplotypes (A lineage) were also found, albeit at lower frequencies, and were mainly concentrated in the Iberian Peninsula (9%). Intensive queen breeding and migratory beekeeping is homogenizing the gene pool of European bee populations. This survey of honey bee maternal diversity across Europe highlights the alarming dominance of C-lineage haplotypes and underscores the importance of conservation apiaries, as they have effectively preserved the autochthonous M-lineage subspecies in different countries. This work was conducted in the framework of the project Better-B, funded by the European Union, the Swiss State Secretariat for Education, Research, and Innovation, and UK Research and Innovation under the UK government's Horizon Europe funding guarantee (grant number 10068544).eng
dc.description.sponsorshipBETTER-B: Improving bees' resilience to stressors by restoring harmony and balance (Nº 101081444)
dc.identifier.citationLi, Fernanda; Lopes, Ana; Costa, Maíra; Henriques, Dora; Quaresma, Andreia; Yadró García, Carlos A.; Albo, Alexandre; Čereškienė, Laima Blažytė; Brodschneider, Robert; Brusbardis, Valters; Carreck, Norman L.; Charistos, Leonidas; Chlebo, Robert; Coffey, Mary F.; Dahle, Bjørn; Danneels, Ellen; Dobrescu, Constantin; Dupleix-Marchal, Anna; Filipi, Janja; Gajda, Anna; Gratzer, Kristina; Groeneveld, Linn Fenna; Hatjina, Fani; Johannesen, Jes; Kolasa, Michal; Körmendy-Rácz, János; Kovačić, Marin; Kristiansen, Preben; Martikkala, Maritta; McCormack, Grace P.; Martín-Hernández, Raquel; Pavlov, Borce; Poirot, Benjamin; Pietropaoli, Marco; Radev, Zheko; Raudmets, Aivar; René-Douarre, Vincent; Roessink, Ivo; Škerl, Maja Ivana Smodiš; Soland, Gabriele; Titera, Dalibor; Steen, Jozef van der; Varnava, Andri; Vejsnæs, Flemming; Fedoriak, Mariia M.; Zarochentseva, Oksana; Webster, Matthew T.; Graaf, Dirk C.; Pinto, Maria Alice (2025). Large Scale Mitochondrial Evidence of C-lineage Dominance in European Honey Bees. In VI International Conference of the Portuguese Society of Genetics. Oeiras
dc.identifier.urihttp://hdl.handle.net/10198/34641
dc.language.isoeng
dc.peerreviewedno
dc.publisherUniversidade Nova de Lisboa
dc.relationBETTER-B: Improving bees' resilience to stressors by restoring harmony and balance (Nº 101081444)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleLarge Scale Mitochondrial Evidence of C-lineage Dominance in European Honey Beeseng
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferenceDate2025
oaire.citation.conferencePlaceOeiras
oaire.citation.endPage67
oaire.citation.startPage67
oaire.citation.titleVI International Conference of the Portuguese Society of Genetics
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLopes
person.familyNameCosta
person.familyNameHenriques
person.familyNameQuaresma
person.familyNameYadró García
person.familyNamePinto
person.givenNameAna
person.givenNameMaíra
person.givenNameDora
person.givenNameAndreia
person.givenNameCarlos A.
person.givenNameM. Alice
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-id291F-986F-07DA
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person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-1888-2262
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person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id57119742600
person.identifier.scopus-author-id8085507800
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