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Advisor(s)
Abstract(s)
A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed, being the best results obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elution with n-hexane/1,4-dioxane (95.5:4.5, v/v) at a flow rate of 0.7 mL/min. The effluent was monitored by a series arrangement of a diode-array followed by a fluorescence detector. All compounds were separated in a short period of time (17 min). The method proved to be rapid, sensitive, reproducible and accurate, allowing the simultaneous determination of all vitamin E isomers.
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Citation
Amaral, J.S.; Casal, S.; Torres, D.; Seabra, R. M.; Oliveira, M.B.P.P. (2005). Simultaneous determination of tocopherols and tocotrienols in hazelnuts by a normal phase liquid chromatographic method. Analytical Sciences. ISSN 0910-6340. 21:12, p. 1545-1548
Publisher
The Japan Society for Analytical Chemistry