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Development of new molecular tools to assess argan oil authenticity: detection of olive oil as a potential adulterant

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dc.contributor.authorRaja, Fatima Zahra
dc.contributor.authorCosta, Joana
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorCharrouf, Zoubida
dc.contributor.authorGrazina, Liliana
dc.contributor.authorVilla, Caterina
dc.contributor.authorKartah, Badr Eddine
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorMafra, Isabel
dc.date.accessioned2020-07-13T16:08:10Z
dc.date.available2020-07-13T16:08:10Z
dc.date.issued2018
dc.description.abstractArgan oil is a traditional product obtained from the argan tree (Arganiaspinosa L.),whichis endemic only to Morocco[1].Both cosmetic and food grade argan oil are commercialized worldwide, attaining very high prices in the international market.For that reason, argan oil is very prone to be adulterated, in particular with cheaper vegetable oils. Therefore, it is important to develop methodologies that can be used in control and inspection programs in order to guarantee argan oil authenticity and quality. In particular, there is the need for methodologies that allow the accurate identification of vegetable oils illegally added to argan oil. The present work aims at developing novel approaches based on DNA markers to detect the presence of adulterants, using olive oil as case study. In silico analysis was performed for the design of Oleaeuropea L. and A. spinosa L. specific primers targeting the chloroplastidialmatKgeneand the ITS2 region, respectively. Samples of authentic argan oil were acquired from a producing cooperativein Morocco, while olive oil samples were obtained from local stores in Portugal. Cross-reactivity was assayed using DNA extracts from other edible and oil producing plant species (n=17). Binary model mixtures were prepared with the addition of known amounts of olive oil in argan oil in the proportions of 50, 25, 5, 1% (w/w), followed by a pre-concentration by centrifugation. DNA was extracted using the Nucleospin Plant kit, protocol B. according to the manufacturer instructions. Specificity and sensitivity of the designed primers were assessed by qualitative PCR. Species-specific PCR assays were successfully developed, producing amplicons of 109 and 117 bp for olive and argan, respectively, down to 0.01 pg of DNA for both species. The application of the olive-specific PCR assay to DNA extracts of binary mixtures enabled the clear detection of 1%. Subsequently, a real-time PCR assay with EvaGreen dye was developed for quantitative analysis using the normalised ΔCq method. The assay confirmed the limit of detection of 1% of olive oil, in a dynamic range of 1-50%, with acceptable correlation coefficient and PCR efficiency (81.1%), considering the type of food matrix. Both, qualitative and quantitative PCR assays can provide simple, fast and high-throughput tools to detect the presence of adulterant oils in argan oil.pt_PT
dc.description.sponsorshipThis work was supported by FCT (Fundação para a Ciência e Tecnologia) through projectsFCT/CNRST (Portugal/Morocco) (FCT/6460/6/6/2017/S), UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by projectNORTE-01-0145-FEDER-000011. J. Costa, L. Grazina and C. Villa are grateful to FCT grants (SFRH/BPD/102404/2014,SFRH/BD/132462/2017 and PD/BD/114576/2016, respectively) financed by POPH-QREN (subsidised by FSE and MCTES).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRaja, F.; Costa, J.; Amaral, J.S.; Charrouf, Z.; Grazina, L.; Villa, C.; Kartah, B.E.; Oliveira, M.B.P.P.; Mafra, I. (2018). Development of new molecular tools to assess argan oil authenticity: detection of olive oil as a potential adulterant. In 1er Congres International de Biotechnologie Verte. Taroudant, Moroccopt_PT
dc.identifier.urihttp://hdl.handle.net/10198/22465
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectArgan oilpt_PT
dc.subjectArgan oil authenticitypt_PT
dc.subjectThe Nucleospin plant kitpt_PT
dc.subjectQualitative PCRpt_PT
dc.subjectSpecies-specific PCRpt_PT
dc.subjectQuantitative PCRpt_PT
dc.titleDevelopment of new molecular tools to assess argan oil authenticity: detection of olive oil as a potential adulterantpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.conferencePlaceTaroudant, Moroccopt_PT
oaire.citation.title1er Congres International de Biotechnologie Vertept_PT
oaire.fundingStream5876
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd
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relation.isProjectOfPublication.latestForDiscovery40f15c30-b6e8-4474-ae1d-d7256c7af84e

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