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Development and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier, 1798) using 454 sequencing

2013Journal article Open access
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dc.contributor.authorFroufe, Elsa
dc.contributor.authorSobral, Carina
dc.contributor.authorTeixeira, Amílcar
dc.contributor.authorLopes, Anabela
dc.contributor.authorSousa, Ronaldo
dc.contributor.authorVarandas, Simone
dc.contributor.authorLopes-Lima, Manuel
dc.date.accessioned2013-09-05T15:55:17Z
dc.date.available2013-09-05T15:55:17Z
dc.date.issued2013
dc.description.abstract1. The Unionidae are among the most endangered fauna in the world and globally in decline. They are particularly vulnerable to habitat loss and fragmentation, susceptible to flow, pollution and climatic disturbances and introduction of invasive species. Despite their well-recognized ecological and conservation importance, there is a surprising lack of genomic resources currently available for European species. 2. The aim of this study was to develop and characterize microsatellites for the near threatened freshwater mussel, Potomida littoralis using 454 sequencing. 3. In order to improve genotyping throughput as well as cost-effectiveness, two multiplex-PCR reactions were designed to amplify 16 new loci. All the new 16 microsatellites were successfully combined in two multiplexed PCR with the number of alleles ranging from 2 to 25 per locus (with a mean of 10), confirming the utility of the new markers. 4. The new genetic markers can therefore be used for studying the population genetic structure and evolution of this species, e.g. to examine current levels of genetic variability within and between populations and thus to contribute to conservation and management.por
dc.identifier.citationFroufe, E.; Sobral, C.; Teixeira, A.; Lopes, A.; Sousa, R.; Varandas S.; Lopes-Lima, M. (2013). Development and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier; 1798) using 454 sequencing. Aquatic Conservation: Marine and Freshwater Ecosystems. ISSN 1099-0755. 23:4, p. 619-623por
dc.identifier.doi10.1002/aqc.2335
dc.identifier.issn1099-0755
dc.identifier.otherDOI: 10.1002/aqc.2335
dc.identifier.urihttp://hdl.handle.net/10198/8639
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relationCONBI: Biodiversity and Conservation of Bivalves - ecogeographic, genetic and physiological information
dc.subjectRiverpor
dc.subjectConservation evaluationpor
dc.subjectNew techniquespor
dc.subjectGeneticspor
dc.subjectInvertebratespor
dc.subjectMolluscspor
dc.titleDevelopment and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier, 1798) using 454 sequencingpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCONBI: Biodiversity and Conservation of Bivalves - ecogeographic, genetic and physiological information
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAAC-AMB%2F117688%2F2010/PT
oaire.citation.titleAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMSpor
oaire.fundingStream5876-PPCDTI
person.familyNameTeixeira
person.givenNameAmilcar
person.identifier2153193
person.identifier.ciencia-id9510-3CF3-0393
person.identifier.orcid0000-0001-5336-1174
person.identifier.scopus-author-id7202385393
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4f0753fd-4b4f-46f6-8e2f-20c7f835a32a
relation.isAuthorOfPublication.latestForDiscovery4f0753fd-4b4f-46f6-8e2f-20c7f835a32a
relation.isProjectOfPublication3984386b-860a-44b4-a2f4-ded6f8a972b6
relation.isProjectOfPublication.latestForDiscovery3984386b-860a-44b4-a2f4-ded6f8a972b6

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