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Morphometric measurements in HPV-transgenic mice after topical application of Cytinus hypocistis (L.) extract

2021Conference object Open access
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dc.contributor.authorSilva, Ana Rita
dc.contributor.authorMedeiros, Catarina
dc.contributor.authorFerreira, Teresa
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorPires, Maria João
dc.contributor.authorCosta, Rui Miguel Gil
dc.contributor.authorGama, Ana
dc.contributor.authorOliveira, Paula A.
dc.date.accessioned2022-01-31T11:32:24Z
dc.date.available2022-01-31T11:32:24Z
dc.date.issued2021
dc.description.abstractHuman papillomavirus (HPV) infections are associated with the development of several anogenital cancers. K14HPV16 transgenic model is an animal model that mimics HPV-induced lesions. Cytinus hypocistis (L.) is an endophytic parasitic plant that has been shown to possess several antibacterial and antiproliferative properties1. During antiproliferative studies in animal models, the toxicological analysis is also required. Therefore, the present research aims to study the toxic effects of applying a topical formulation enriched with Cytinus hypocistis extracts (FCH) on K14HPV16 mice. Three different concentrations of FCH were used to enrich a base cream, the phenolic compounds were analyzed by HPLC-DAD-ESI/MS and were applied to the animal’s ears for 28 days. Thirty female mice were equally divided into six groups (G) (n=5): G1 (HPV16+-C1); G2 (HPV16+-C2); G3 (HPV16+-C3); G4 (HPVC3); G5 (HPV+-control); and G6 (HPV-control). The animals were kept under controlled conditions and biological variables were registered throughout the study. In the end, animals were sacrificed, and the organs collected and weighed. The main phenolic compound present in the base cream enriched with C. hypocistis was galloyl-bis-HHDPglucose. Food and water intake were constant throughout the trial. G3 presented a higher relative weight of the liver and lungs than the control animals, and a significant difference in body weight relatively to G4 (p<0.05). The body weight loss and the increase in liver weight observed in HPV+ mice treated with they may reflect a negative impact of the extract on these already fragile animals. However, overall, the FCH was well tolerated by the animals. Although the highest concentration of the extract had a negative impact on the evaluated morphometric parameters, this does not necessarily imply that the extract is toxic, which can be corroborated with histological analysis. Further studies are needed to understand whether different doses of this extract would cause distinct effects.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). A. R. Silva is grateful to FCT for her Doctoral Grant (SFRH/BD/145834/2019) and L. Barros for her contract through the institutional scientific employment program.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Ana Rita; Medeiros, Catarina; Ferreira, Teresa; Barros, Lillian; Ferreira, Isabel C.F.R.; Pires, Maria J.; Costa, Rui Gil da; Gama, Ana; Oliveira, Paula A. (2021). Morphometric measurements in HPV-transgenic mice after topical application of Cytinus hypocistis (L.) extract. In II Symposium on Applied Biochemistry. ISBN 978-989-98830-1-7
dc.identifier.isbn978-989-98830-1-7
dc.identifier.urihttp://hdl.handle.net/10198/24962
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleMorphometric measurements in HPV-transgenic mice after topical application of Cytinus hypocistis (L.) extractpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleCytinus hypocistis L. L.: a case study for the development of an innovative, safe, and effective anti-photoaging cosmeceutical formulation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F145834%2F2019/PT
oaire.citation.conferencePlaceonline editionpt_PT
oaire.citation.titleII Symposium on Applied Biochemistrypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameSilva
person.familyNameBarros
person.familyNameFerreira
person.givenNameAna Rita
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierABD-5857-2020
person.identifier469085
person.identifier144781
person.identifier.ciencia-id0E1A-35C1-C79A
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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