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High pH reversed-phase preparative chromatographic separation of nadolol racemates using C18 adsorbents

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dc.contributor.authorArafah, Rami
dc.contributor.authorRibeiro, António E.
dc.contributor.authorRodrigues, Alírio
dc.contributor.authorPais, Luís S.
dc.date.accessioned2020-06-15T16:15:34Z
dc.date.available2020-06-15T16:15:34Z
dc.date.issued2018
dc.description.abstractIn recent years, the continuous improvement of preparative liquid chromatographic techniques, make easier the resolution of complex multicomponent chiral drugs, into single pure stereoisomers, by means of combining different strategies and using chiral and achiral adsorbents. Nadolol is one representative beta-blocker pharmaceutical drug prescribed worldwide for relieve of several diseases related with the cardiovascular system. This pharmaceutical chiral drug represents a very interesting case-study since it is composed by four stereoisomers, being a mixture of two racemates, i.e., a mixture of two pairs of enantiomers. The complete separation of all the four nadolol stereoisomers can be achieved using alternative strategies, different types of separation sequences and techniques, such as, the use of different adsorbents (chiral and achiral stationary phases), and the correspondent mobile phase optimization at both normal and reversed-phase modes [1-3]. In this work, a large set of experimental results will be presented for the separation of the two nadolol racemates using a commercial Azura preparative HPLC system, equipped with two 250 mL/min pump heads. The fixed-bed separation was carried out through a sequence of multiple injections, optimized by taking into account the retention time of both racemates using an XBridge Prep OBD C18 column with preparative dimensions (250mm ID x 30mm L) and with a particle size diameter of 10 m [4]. Experimental results will show the optimization of the mobile phase composition and injection time. The experimental results presented in this work stresses out the advantage of using a first achiral reversed-phase chromatographic separation step to perform the separation of the two nadolol racemates.pt_PT
dc.description.sponsorshipAIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable Northern Region of Portugal 2020”, with the reference NORTE-01-0145-FEDER-000006, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and of project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM funded by ERDF through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e Tecnologia.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationArafah, Rami; Ribeiro, António E.; Rodrigues, A.E.; Pais, L.S. (2018). High pH reversed-phase preparative chromatographic separation of nadolol racemates using C18 adsorbents. In Livro de Resumos do XXIV Encontro Luso-Galego de Quimica. ISBN 978-989-8124-24-1pt_PT
dc.identifier.isbn978-989-8124-24-1
dc.identifier.urihttp://hdl.handle.net/10198/22057
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Portuguesa de Químicapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectNadolol racematespt_PT
dc.subjectChiral and achiralpt_PT
dc.titleHigh pH reversed-phase preparative chromatographic separation of nadolol racemates using C18 adsorbentspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePorto, Portugalpt_PT
person.familyNameArafah
person.familyNameRibeiro
person.familyNamePais
person.givenNameRami
person.givenNameAntónio E.
person.givenNameLuís S.
person.identifier.ciencia-idAB16-ECBF-B886
person.identifier.ciencia-id3211-D5B0-870D
person.identifier.ciencia-id421E-0CCD-A84F
person.identifier.orcid0000-0002-7743-4988
person.identifier.orcid0000-0003-4569-7887
person.identifier.orcid0000-0002-3000-2862
person.identifier.scopus-author-id57151342900
person.identifier.scopus-author-id23390701300
person.identifier.scopus-author-id6603930478
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery3cd4864a-8aa6-46a2-9415-767a7551ee62

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