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Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS

2015Journal article Open access
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dc.contributor.authorGarcia, Juliana
dc.contributor.authorOliveira, Ana Paula Leandro
dc.contributor.authorPinho, P. Guedes de
dc.contributor.authorFreitas, Victor
dc.contributor.authorCarvalho, Alexandra T.P.
dc.contributor.authorBaptista, Paula
dc.contributor.authorPereira, Eric Carvalho
dc.contributor.authorBastos, Maria de Lourdes
dc.contributor.authorCarvalho, Félix
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-01T16:05:04Z
dc.date.accessioned2018-03-05T09:18:56Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-01T16:05:04Z
dc.date.available2018-03-05T09:18:56Z
dc.date.issued2015
dc.description.abstractAmanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.en_EN
dc.description.sponsorshipAuthors are grateful to Dr Zélia dos Santos Azevedo, Faculty of Sciences, University of Porto, who loaned LC/DAD-ESI/MS and for all technical assistance. The authors also are grateful for the help of the Foundation for the Science and Technology (FCT, Portugal) for financial support and also thank FCT for doctoral grant SFRH/BD/74979/2010.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationGarcia, Juliana; Oliveira, Ana; Guedes de Pinho, Paula; Freitas, Victor; Carvalho, Alexandra; Baptista, Paula; Pereira, Eric; Bastos, Maria de Lourdes; Carvalho, Félix (2015). Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS. Mycologia. ISSN 0027-5514. 107, p. 679-687en_EN
dc.identifier.doi10.3852/14-253en_EN
dc.identifier.issn0027-5514
dc.identifier.urihttp://hdl.handle.net/10198/16134
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAmanita phalloidesen_EN
dc.subjectMass spectrometryen_EN
dc.subjectReversed-phase high performance liquid chromatographyen_EN
dc.subjectToxinsen_EN
dc.subjectα-amanitinen_EN
dc.titleDetermination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MSen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F74979%2F2010/PT
oaire.fundingStreamSFRH
person.familyNameBaptista
person.givenNamePaula
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0001-6331-3731
person.identifier.scopus-author-id14051688000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isAuthorOfPublication.latestForDiscovery3f35226a-b17a-4f7d-8da1-3297105cbfe9
relation.isProjectOfPublication3140ff16-4042-4a9f-bf62-fa6a3fea5229
relation.isProjectOfPublication.latestForDiscovery3140ff16-4042-4a9f-bf62-fa6a3fea5229

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