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Extractability of rosmarinic acid by using three different aqueous based extraction procedures

dc.contributor.authorCarocho, Márcio
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2018-02-14T10:51:42Z
dc.date.available2018-02-14T10:51:42Z
dc.date.issued2017
dc.description.abstractRosmarinic acid is the main constituent of the rosemary extract (E392), which is the only natural extract allowed in the European Union to be used as food preservative. Furthermore, rosmarinic acid has many bioactivities, namely astringent, antioxidative, anti-inflammatory, antimutagenic, antibacterial and antiviral. It is also used against dyspepsia, dysmenorrhea, rheumatic diseases and Herpes simples, among others [1]. Various techniques can be employed to extract compounds from different plant parts, including traditional extraction protocols through maceration, as also extractions assisted by microwaves and supercritical fluids, among others. In this study, four different plant species were submitted to aqueous based extractions in order to determine the best conditions to obtain the highest quantity of rosmarinic acid. Rosemary (Rosmarinus officinalis L.) was chosen due to its permission to be used in the food industry and also for its high content of rosmarinic acid. Basil (Ocimum basilicum L.), Oregano (Origanum vulgare L.), and Sage (Salvia officinalis L.) were chosen for being putative sources of natural food preservatives in the near future, having also interesting bioactivities, no toxicity and a fair amount of rosmarinic acid in relation to rosemary [2]. The three chosen aqueous extraction systems were infusion, decoction and hydroethanol extraction (80:20, v/v). All the extracts were analysed using a High- Performance Liquid Chromatography coupled to a Diode Array Detector and an Electrospray Ionization Mass Spectrometer (HPLC-DAD-ESI/MS). Table 1 shows the amount of rosmarinic acid in each extraction conditions (mg/g). It is clear that infusion and hydroethanol extractions are best suited to obtain rosmarinic acid. Infusions proved to be more suitable in the cases of rosemary and oregano, while the hydroethanol mixture extracted higher contents in basil and sage. This study highlights infusion as the most adequate extraction procedure to obtain rosmarinic acid, a compound with great interest in the food industry.pt_PT
dc.description.sponsorshipFCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013). Project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT. FEEI - North 2020, for financial support within the scope of the Projects NORTE-01-0145-FEDER-023289: DeCodE and Mobilizador ValorNatural®, as also to Interreg España-Portugal through the project 0377_Iberphenol_6_E.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarocho, Márcio; Barros, Lillian; Ferreira, Isabel C.F.R. (2017). Extractability of rosmarinic acid by using three different aqueous based extraction procedures. In 10º Encontro Nacional de Cromatografia. Bragança. ISBN 978-972-745-234-7pt_PT
dc.identifier.isbn978-972-745-234-7
dc.identifier.urihttp://hdl.handle.net/10198/15716
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Bragançapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleExtractability of rosmarinic acid by using three different aqueous based extraction procedurespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.title10º Encontro Nacional de Cromatografiapt_PT
oaire.fundingStream5876
person.familyNameCarocho
person.familyNameBarros
person.familyNameFerreira
person.givenNameMárcio
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierawbWiiMAAAAJ&hl
person.identifier469085
person.identifier144781
person.identifier.ciencia-id5614-005B-5CF1
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id52463229800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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