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Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth

2021Journal article Open access
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dc.contributor.authorHaile, Tesfay
dc.contributor.authorCardoso, Susana M.
dc.contributor.authorRaphaelli, Chirle de Oliveira
dc.contributor.authorPereira, Olívia R.
dc.contributor.authorPereira, Elisa dos Santos
dc.contributor.authorVizzotto, Márcia
dc.contributor.authorNora, Leonardo
dc.contributor.authorAsfaw, Adissu Alemayehu
dc.contributor.authorPeriasamy, Gomathi
dc.contributor.authorKarim, Aman
dc.date.accessioned2021-06-15T14:09:03Z
dc.date.available2021-06-15T14:09:03Z
dc.date.issued2021
dc.description.abstractThymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH•), hydroxyl (•OH), and nitric oxide (•NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for •NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 = 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents.pt_PT
dc.description.sponsorshipThis study was supported by a financial grant obtained from MU-NMBU project of Mekelle University (PG/MSc/MU-NMBU/CHS/55/2011) for data collection only. Authors acknowledge Mekelle University, Ethiopia for providing necessary facilities and also thank Getinet Masresha, Department of Biology, University of Gondar, Ethiopia for authenticating the plant sample. Thanks are due to University of Aveiro, FCT/ MEC for the financial support to the LAQV-REQUIMTE (UIDB/50006/2020) and CIMO (UIDB/00690/2020), through national funds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement. SC thanks the research contract under the project AgroForWealth (CENTRO-01–0145-FEDER-000001), funded by Centro 2020, through FEDER and PT 2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHaile, Tesfay; Cardoso, Susana M.; Raphaelli, Chirle de Oliveira; Pereira, Olívia R.; Pereira, Elisa dos Santos; Vizzotto, Márcia; Nora, Leonardo; Asfaw, Adissu Alemayehu; Periasamy, Gomathi; Karim, Aman (2021). Chemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benth. Frontiers in Pharmacology. ISSN 1663-9812. 12, p. 1-14pt_PT
dc.identifier.doi10.3389/fphar.2021.621536pt_PT
dc.identifier.issn1663-9812
dc.identifier.urihttp://hdl.handle.net/10198/23656
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectEssential oilpt_PT
dc.subjectThyme-decoctionpt_PT
dc.subjectAlpha-amylase,pt_PT
dc.subjectThymus serrulatuspt_PT
dc.subjectAlphaglucosidasept_PT
dc.subjectPhenolic compounds profilept_PT
dc.subjectAntihyperglycemic activitypt_PT
dc.titleChemical composition, antioxidant potential, and blood glucose lowering effect of aqueous extract and essential oil of Thymus Serrulatus Hochst. Ex Benthpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage621536pt_PT
oaire.citation.titleFrontiers in Pharmacologypt_PT
oaire.citation.volume12pt_PT
person.familyNamePereira
person.givenNameOlívia R.
person.identifier145216
person.identifier.ciencia-idB312-AF21-D2FA
person.identifier.orcid0000-0002-6275-3134
person.identifier.ridH-1049-2014
person.identifier.scopus-author-id52264387000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8fdab9a-3ad6-47e6-b90c-7a37aabcb453
relation.isAuthorOfPublication.latestForDiscoverye8fdab9a-3ad6-47e6-b90c-7a37aabcb453

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