Repository logo
 
Publication

A lab-made e-nose-MOS device for assessing the bacterial growth in a solid culture medium

2023Journal article Open access
Simple item page
dc.contributor.authorDias, Teresa
dc.contributor.authorSantos, Vitor S.
dc.contributor.authorZorgani, Tarek
dc.contributor.authorFerreiro, Nuno Manuel
dc.contributor.authorRodrigues, Ana Isabel
dc.contributor.authorZaghdoudi, Khalil
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorPeres, António M.
dc.date.accessioned2014-02-12T13:10:57Z
dc.date.available2014-02-12T13:10:57Z
dc.date.issued2023
dc.description.abstractThe detection and level assessment of microorganisms is a practical quality/contamination indicator of food and water samples. Conventional analytical procedures (e.g., culture methods, immunological techniques, and polymerase chain reactions), while accurate and widely used, are time-consuming, costly, and generate a large amount of waste. Electronic noses (E-noses), combined with chemometrics, provide a direct, green, and non-invasive assessment of the volatile fraction without the need for sample pre-treatments. The unique olfactory fingerprint generated during each microorganism’s growth can be a vehicle for its detection using gas sensors. A lab-made E-nose, comprising metal oxide semiconductor sensors was applied, to analyze solid medium containing Gram-positive (Enterococcus faecalis and Staphylococcus aureus) or Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The electrical-resistance signals generated by the E-nose coupled with linear discriminant analysis allowed the discrimination of the four bacteria (90% of correct classifications for leave-one-out cross-validation). Furthermore, multiple linear regression models were also established allowing quantifying the number of colony-forming units (CFU) (0.9428 ≤ R2 ≤ 0.9946), with maximum root mean square errors lower than 4 CFU. Overall, the E-nose showed to be a powerful qualitative–quantitative device for bacteria preliminary analysis, being envisaged its possible application in solid food matrices.por
dc.identifier.citationDias, Teresa; Santos, Vitor S.; Zorgani, Tarek; Ferreiro, Nuno Manuel; Rodrigues, Ana Isabel; Zaghdoudi, Khalil; Veloso, Ana C.A.; Peres, António M.. (2023). A lab-made e-nose-MOS device for assessing the bacterial growth in a solid culture medium. Biosensors. eISSN 2079-6374. 13:1, p. 1-13por
dc.identifier.doi10.3390/bios13010019
dc.identifier.eissn2079-6374
dc.identifier.urihttp://hdl.handle.net/10198/9307
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherMDPIpor
dc.subjectElectronic nosepor
dc.subjectMetal oxide semiconductor sensorspor
dc.subjectBacteria identification
dc.subjectGram-positive
dc.subjectGram-negative
dc.subjectColony-forming units
dc.subjectLinear discriminant analysis
dc.subjectMultiple linear regression models
dc.subjectSimulated annealing algorithm
dc.titleA lab-made e-nose-MOS device for assessing the bacterial growth in a solid culture mediumpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiosensorspor
person.familyNameDias
person.familyNamePeres
person.givenNameTeresa
person.givenNameAntónio M.
person.identifier142703
person.identifier107333
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id7102331969
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication87c9cae0-314d-4622-ba85-4d3ec5726606
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery7d93be47-8dc4-4413-9304-5b978773d3bb

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
A lab-made e-nose-MOS.pdf
Size:
1.11 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.73 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus