Repository logo
 
Publication

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

2014Journal article Open access
Simple item page
dc.contributor.authorMartins, Ivone
dc.contributor.authorMartins, Fátima
dc.contributor.authorBelo, Hélio
dc.contributor.authorVaz, Madalena
dc.contributor.authorCarvalho, Marisa
dc.contributor.authorCravador, Alfredo
dc.contributor.authorChoupina, Altino
dc.date.accessioned2014-09-22T16:07:20Z
dc.date.available2014-09-22T16:07:20Z
dc.date.issued2014
dc.description.abstractOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamomi. In this work we report the sequencing and characterization of a class of GIPs secreted by Phytophthora cinnamomi. The gip gene from P. cinnamomi has a 937 bp ORF encoding a putative peptide of 312 deduced amino acids. The expression of this gene was studied during growth in different carbon sources (glucose, cellulose and sawdust), by RT-qPCR and its level of expression was evaluated at five time points. The highest expression of gip gene occurred in sawdust at 8 h of induction. In vivo infection of C. sativa revealed an increase in gip expression from 12 to 24 h. At 36 h its expression decreased suggesting that a compensatory mechanism must occur in plant.por
dc.identifier.citationMartins, Ivone; Martins, Fátima; Belo, Hélio; Vaz, Madalena; Carvalho, Marisa; Cravador, Alfredo; Choupina, Altino (2014). Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi. Molecular Biology Reports. ISSN 0301-4851. 41:4, p. 2453-2462por
dc.identifier.doi10.1007/s11033-014-3101-1
dc.identifier.issn0301-4851
dc.identifier.urihttp://hdl.handle.net/10198/10537
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectCastanea sativapor
dc.subjectGIPpor
dc.subjectHE-TAIL PCRpor
dc.subjectPhytophthora cinnamomipor
dc.subjectRT-qPCRpor
dc.titleCloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomipor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT
oaire.citation.endPage2462por
oaire.citation.issue4por
oaire.citation.startPage2453por
oaire.citation.titleMolecular Biology Reportspor
oaire.citation.volume41por
oaire.fundingStream3599-PPCDT
person.familyNameChoupina
person.givenNameAltino
person.identifier587972
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication.latestForDiscovery314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isProjectOfPublication5169eaef-93f4-4316-9542-d2a31ebb44d8
relation.isProjectOfPublication.latestForDiscovery5169eaef-93f4-4316-9542-d2a31ebb44d8

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Gip p. cinnamomi publicação.pdf
Size:
724.3 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus