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Plant food supplements (PFS) for weight-loss are very popular, being among the most-well
consumed. The aim of this study was to detect Citrus aurantium in PFS, using polymerase chain
reaction (PCR)-based methods. For this purpose, teas labelled as containing C. aurantium were
commercially acquired. Voucher leaves of C. aurantium and other Citrus spp. (C. sinensis, C. limon,
C. medica) were gently provided by Germplasm Banks. Primers were specifically designed in three
different genomic sequences, namely RGAs4-6-like gene that encodes a resistance-like protein and
two anonymous marker genes of C. aurantium retrieved from NCBI database. DNA was extracted
using the commercial Nucleospin Plant 11 kit. Yield, purity and integrity of extracts were evaluated by
UV /Vis spectrophotometry and agarose gel electrophoresis.
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Citação
Sousa, Inês; Costa, Joana; Fernandes, Telmo; Amaral, J.S.; Oliveira, M.B.P.P.; Mafra, Isabel (2016). Development of a polymerase chain reaction assay for the specific detection of Citrus aurantium in plant food supplements. In 9th Edition of Young Investigators Meeting from University of Porto: book of abstracts. Porto