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Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole

2014Journal article Open access
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dc.contributor.authorFaria-Ramos, Isabel
dc.contributor.authorFarinha, Sofia
dc.contributor.authorNeves-Maia, João
dc.contributor.authorTavares, Pedro Ribeiro
dc.contributor.authorMiranda, Isabel M.
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorPina-Vaz, Cidália
dc.contributor.authorRodrigues, Acácio G.
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2018-03-08T15:14:43Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2018-03-08T15:14:43Z
dc.date.issued2014
dc.description.abstractBackground: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatus was performed according to the CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation of susceptible A. fumigatus isolates, at 35°C and 180 rpm, in fresh GYEP broth medium supplemented with Prochloraz (PCZ), a potent agricultural antifungal, for a period of 30 days. Minimal inhibitory concentrations (MIC) of PCZ and clinical azoles were monitored every ten days. In order to assess the stability of the developed MIC, the strains were afterwards sub-cultured for an additional 30 days in the absence of antifungal. Along the in vitro induction process, microscopic and macroscopic cultural observations were registered. Results: MIC of PCZ increased 256 times after the initial exposure; cross-resistance to all tested clinical azoles was observed. The new MIC value of agricultural and of clinical azoles maintained stable in the absence of the selective PCZ pressure. PCZ exposure was also associated to morphological colony changes: macroscopically the colonies became mostly white, losing the typical pigmentation; microscopic examination revealed the absence of conidiation. Conclusions: PCZ exposure induced Aspergillus fumigatus morphological changes and an evident increase of MIC value to PCZ as well as the development of cross-resistance with posaconazole, itraconazole and voriconazole.en_EN
dc.description.sponsorshipIFR and IMM are supported by FCT (Fundação Ciência e Tecnologia). IFR is supported by FCT PhD grant (SFRH/BD/91155/2012). I.MM is supported by FCT, Ciência 2008 and co-financed by the European Social Fund. The authors would like to thank Isabel Santos for the excellent technical assistance. Part of the results was presented at the 52nd Interscience Conference on Antimicrobial Agents and Chemotherapy, ICAAC, held San Francisco, USA, September 2012.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFaria-Ramos, Isabel; Farinha, Sofia; Neves-Maia, João; Tavares, Pedro Ribeiro; Miranda, Isabel M.; Estevinho, Letícia M.; Pina-Vaz, Cidália; Rodrigues, Acácio G. (2014). Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole. BMC Microbiology. ISSN 1471-2180. 14, p. 1-5en_EN
dc.identifier.doi10.1186/1471-2180-14-155en_EN
dc.identifier.urihttp://hdl.handle.net/10198/16212
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationEMERGENCE OF MULTIDRUG CROSS-RESISTANCE BETWEEN AGRICULTURAL AND HUMAN ANTIFUNGALS IN CLINICALLY RELEVANT SPECIES OF ASPERGILLUS AND CANDIDA
dc.subjectAspergillus fumigatusen_EN
dc.subjectClinical and agricultural azolesen_EN
dc.subjectCross-resistanceen_EN
dc.titleDevelopment of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azoleen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEMERGENCE OF MULTIDRUG CROSS-RESISTANCE BETWEEN AGRICULTURAL AND HUMAN ANTIFUNGALS IN CLINICALLY RELEVANT SPECIES OF ASPERGILLUS AND CANDIDA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F91155%2F2012/PT
person.familyNameEstevinho
person.givenNameLetícia M.
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.orcid0000-0002-9249-1948
person.identifier.scopus-author-id6506577664
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublication.latestForDiscovery4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isProjectOfPublication37bb0d78-d188-430c-b08b-ed24f8453a3f
relation.isProjectOfPublication.latestForDiscovery37bb0d78-d188-430c-b08b-ed24f8453a3f

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