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Advisor(s)
Abstract(s)
Olive mill wastewaters (OMW) have been attracting the attention of food and
pharmaceutical industries, as it is a potential source of biophenols with a wide
range of biological activities. This effluent produced in the olive oil industry
contains approximately half of the total phenolic compounds of the olive fruit and
it is currently exploited for industrial extraction of hydroxytyrosol [1]. Still, the
phenolic composition of OMW is extremely complex, and many compounds are
yet unidentified. In this context, the identification of unknown phenolic
compounds can encourage the search of new bioactive compounds in OMW and
contribute to further valorize this waste.
In the present work, six new phenolic compounds were identified in Portuguese
olive mill wastewaters. The phenolic extracts of OMW were obtained by methanol
and were fractionated on Sep Pack C18 cartridges, by elution of the cartridges
with 50% (v/v) methanol. The analysis of the purified fractions by electrospray
mass spectrometry in the negative mode showed high relative abundant [M-H]−
ions at m/z 539 and m/z 523, corresponding respectively to oleuropein and
ligstroside isomers that contain the glucose unit linked to its hydroxytyrosol
moiety. Glucoside derivatives of these compounds were also found in the two
OMW samples. In particular, the fragmentation pathway of the [M-H]− ion at m/z
863 demonstrated the presence of a diglucoside derivative of the oleuropein
isomer, while those of the ions at m/z 685 and m/z 847 were consistent with
mono- and a di-glucosides of the ligstroside isomer, respectively. Moreover, the structure of an elenoic derivative of the ion at m/z 685 was also elucidated. Studies regarding the abundance and the biological activities of these compounds are now required to determine their possible industrial exploitation.
[1] Agallias A., Magiatis P., Skaltsounis A.-P., Mikros E., Tsarbopoulos A., Gikas E., Spanos I., Manios T. (2007). J. Agric. Food Chem. 55: 2671-2676.
Description
Keywords
Phenolic compounds Olive mill wastewaters Oleuropein Ligstroside
Citation
Cardoso, Susana M.; Falcão, Soraia I.; Peres, António M.; Domingues, M. R. M. (2010). New biophenols in olive mill wastewaters. In 2010 EFFoST Annual Meeting - Food and Health. Dublin