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Electrospun polycaprolactone membranes functionalized with nanochitin for enhanced bioactivity in localized cancer photodynamic therapy

2025Journal article Open access
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datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosCiências Naturais::Outras Ciências Naturais
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCosta, Sofia M.
dc.contributor.authorMattos, Bruno D.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorZhu, Ya
dc.contributor.authorLima, Eurico
dc.contributor.authorReis, Lucinda
dc.contributor.authorRojas, Orlando J.
dc.contributor.authorFangueiro, Raul
dc.contributor.authorFerreira, Diana P.
dc.date.accessioned2025-11-11T16:02:02Z
dc.date.available2025-11-11T16:02:02Z
dc.date.issued2025
dc.description.abstractThe encapsulation of photosensitizers (PSs) in electrospun membranes has emerged as a promising approach in photodynamic therapy (PDT) on tumor sites, overcoming the drawbacks associated with systemic administration. In this work, localized implants for cancer treatment using PDT were developed by incorporating EL-2 squaraine into poly(epsilon-caprolactone) (PCL) electrospun microfibers. The latter were coated with chitin nanocrystals (ChNC) by electrospraying, which may improve the biocompatibility and bioactivity of the developed membranes, potentially enhancing the clinical outcomes. The developed electrospun membranes were characterized by water contact angle, imaging, and spectroscopy techniques. The uniform encapsulation and distribution of EL-2 within the microfibers were confirmed while ChNC endowed the membranes with surface hydrophilicity. EL-2 alone displayed about 20 times more cytotoxicity after irradiation compared to the dark condition against HeLa cervical carcinoma cells. Meanwhile, the photodynamic action of PCL+EL-2/ChNC membranes promoted a significant inhibition of cancer cells' proliferation under irradiation, achieving 66.25 % of inhibition, compared to only 24.78 % in dark conditions, using the highest concentration of EL-2. Overall, this work introduces a disruptive strategy using electrospinning-electrospraying to design fibrous therapeutic platforms for cancer PDT, taking advantage of electrospun fibers unique features and the localized nature of photodynamic therapy.eng
dc.description.sponsorshipThis work was funded by the European Regional Development Fund through the Operational Competitiveness Program and the National Foundation for Science and Technology of Portugal (FCT) under the projects UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T) on its components Base (https://doi.org/10.54499/UIDB/00264/2020) and programmatic (https://doi.org/10.54499/UIDP/00264/2020). The authors are also thankful to the projects UID/QUI/UI0616/2019 – Chemistry Centre of Vila Real, funded by National Founds through FCT/MCTES - Fundação para a Ciência e a Tecnologia. Sofia M. Costa and Eurico Lima are thankful for the FCT PhD Scholarships SFRH/BD/147517/2019 and SFRH/BD/147645/2019, respectively. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020); the contract of RC Cal-helha through the institutional scientific employment program-contract (CEEC-INST; DOI:10.54499/CEECINST/00016/2018/CP1505/CT0009).
dc.identifier.citationCosta, Sofia M.; Mattos, Bruno D.; Zhu, Ya; Lima, Eurico; Reis, Lucinda; Rojas, Orlando J.; Fangueiro, Raul; Ferreira, Diana P. (2025). Electrospun polycaprolactone membranes functionalized with nanochitin for enhanced bioactivity in localized cancer photodynamic therapy. Carbohydrate Polymer Technologies and Applications. ISSN 2666-8939. 11, p. 1-12
dc.identifier.doi10.1016/j.carpta.2025.100895
dc.identifier.issn2666-8939
dc.identifier.urihttp://hdl.handle.net/10198/35044
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre for Textile Science and Technology
dc.relationLocal drug delivery systems based on biodegradable nanofibers for cancer photodynamic therapy PDT
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.relation.ispartofCarbohydrate Polymer Technologies and Applications
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectElectrospinning
dc.subjectElectrospraying
dc.subjectLocalized drug delivery systems
dc.subjectBiodegradable polymers
dc.subjectCancer photodynamic therapy
dc.titleElectrospun polycaprolactone membranes functionalized with nanochitin for enhanced bioactivity in localized cancer photodynamic therapyeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Textile Science and Technology
oaire.awardTitleLocal drug delivery systems based on biodegradable nanofibers for cancer photodynamic therapy PDT
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00264%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147517%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage12
oaire.citation.startPage1
oaire.citation.titleCarbohydrate Polymer Technologies and Applications
oaire.citation.volume11
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCalhelha
person.givenNameRicardo C.
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.orcid0000-0002-6801-4578
person.identifier.ridJ-2172-2014
person.identifier.scopus-author-id6507978333
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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