Repository logo
 
Publication

Development of an electrochemical RNA-aptasensor to detect human osteopontin

2015Journal article Open access
Simple item page
dc.contributor.authorMeirinho, Sofia G.
dc.contributor.authorDias, L.G.
dc.contributor.authorPeres, António M.
dc.contributor.authorRodrigues, Lígia R.
dc.date.accessioned2015-05-11T14:29:29Z
dc.date.available2015-05-11T14:29:29Z
dc.date.issued2015
dc.description.abstractElectrochemical aptasensors may be used to detect protein biomarkers related to tumor activity. Osteopontin (OPN), a protein present in several body fluids, has been suggested as a potential biomarker since its overexpression seems to be associated with breast cancer progression and metastasis. In this work, a simple and label-free voltammetric aptasensor for the detection of OPN, using an RNA aptamer previously reported to have affinity for human OPN as the molecular recognition element, and the ferro/ferricyanide solution as a redox probe, was developed. The RNA aptamer was synthetized and immobilized in a working microelectrode gold surface (diameter of 0.8 mm) of a screen-printed strip with a silver pseudo-reference electrode and a gold counter electrode. The electrochemical behavior of the electrode surface after each preparation step of the aptasensor was studied using cyclic voltammetry and square wave voltammetry. The resulting voltammetric aptasensor was used to detect OPN in standard solutions. Cyclic voltammetry results showed that the aptasensor has reasonable detection and quantification limits(3.7+-0.6 nM and 11+-2 nM, respectively). Indeed, the detection limit falls within the osteopontin levels reported in the literature for patients with metastatic breast cancer. Moreover, the aptasensor is able to selectively detect the target protein in the presence of other interfering proteins, except for thrombin. Considering the overall results, a possible application of the aptasensor for cancer prognosis may be foreseen in a near future.por
dc.description.sponsorshipThis workwasfundedbyFundaçãoparaaCiênciaeaTecno- logia (FCT)throughthePhDgrantSRFH/BD/65021/2009.More- over,theworkwasco-financed byFCTandFEDERunderProgram PT2020 (ProjectUID/EQU/50020/2013);bytheStrategicProject PEst-OE/EQB/LA0023/2013andbytheprojectref.RECI/BBB-EBI/ 0179/2012(projectnumberFCOMP-01-0124-FEDER-027462)fun- ded byFCT.
dc.identifier.citationMeirinho, S.G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2014). Development of an electrochemical RNA-aptasensor to detect human osteopontin. Biosensors and Bioelectronics. ISSN 0956-5663. 71, p. 332-341por
dc.identifier.doi10.1016/j.bios.2015.04.050
dc.identifier.issn0956-5663
dc.identifier.urihttp://hdl.handle.net/10198/11812
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationDEVELOPMENT OF A NOVEL APTAMER-BASED MULTI-SENSOR DEVICE FOR THE DETECTION OF OSTEOPONTIN
dc.subjectRNA aptamerpor
dc.subjectVoltammetric aptasensorpor
dc.subjectOsteopontinpor
dc.subjectProtein biomarkerpor
dc.titleDevelopment of an electrochemical RNA-aptasensor to detect human osteopontinpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF A NOVEL APTAMER-BASED MULTI-SENSOR DEVICE FOR THE DETECTION OF OSTEOPONTIN
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F65021%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F50020%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/RECI%2FBBB-EBI%2F0179%2F2012/PT
oaire.citation.endPage342por
oaire.citation.startPage332por
oaire.citation.titleBiosensors and Bioelectronicspor
oaire.citation.volume71por
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamCOMPETE
person.familyNameDias
person.familyNamePeres
person.givenNameLuís G.
person.givenNameAntónio M.
person.identifier107333
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-1210-4259
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id23569169900
person.identifier.scopus-author-id7102331969
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscoveryeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isProjectOfPublication00a2d49b-e8a8-4563-8ce8-08193b5187fb
relation.isProjectOfPublication66ec3fc3-043e-4147-a64b-3d87442cc076
relation.isProjectOfPublicationb5c9b3e7-c7a3-4d38-8d0f-12f007818bbf
relation.isProjectOfPublicationaa337250-fcb4-43bb-987c-4cd1094f7012
relation.isProjectOfPublication.latestForDiscovery00a2d49b-e8a8-4563-8ce8-08193b5187fb

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2015_Biosensors and Bioelectronics.pdf
Size:
727.53 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus