Publication
Quantitative risk assessment of Listeria monocytogenes in traditional Minas cheeses: the cases of artisanal semi-hard and fresh soft cheeses
| dc.contributor.author | Campagnollo, Fernanda B. | |
| dc.contributor.author | Gonzales-Barron, Ursula | |
| dc.contributor.author | Cadavez, Vasco | |
| dc.contributor.author | Sant'Ana, Anderson S. | |
| dc.contributor.author | Schaffner, Donald W. | |
| dc.date.accessioned | 2018-02-19T10:00:00Z | |
| dc.date.accessioned | 2018-07-31T15:22:40Z | |
| dc.date.available | 2018-01-19T10:00:00Z | |
| dc.date.available | 2018-07-31T15:22:40Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | This study estimated the risk of listeriosis from Brazilian cheese consumption using quantitative microbial risk assessment (QMRA). Risks associated to consumption of two cheese types were assessed: artisanal ripened semihard cheese (produced with raw milk) and refrigerated fresh soft cheese (produced with pasteurized milk). The semi-hard cheese model predicted Listeria monocytogenes growth or decline during ripening, while the soft cheese model predicted pathogen growth during refrigerated storage. Semi-hard cheese modeling scenarios considered L. monocytogenes starting concentration from −2.4 to 6 log CFU/mL in raw milk and three ripening times (4, 22 and 60 days). Soft cheese modeling scenarios considered L. monocytogenes starting concentration from −2.4 to 4 log CFU/mL in milk. The inclusion of anti-listerial lactic acid bacteria (LAB) in cheeses was also examined. Risk of listeriosis due to consumption of soft cheese was 6000 and 190 times greater than that of semi-hard cheese, for general and vulnerable populations, respectively. Aging semi-hard cheese reduced risk, and risk was influenced by L. monocytogenes starting concentration. Aging cheese with inhibitory LAB for 22 days reduced risk over 4 million-fold when L. monocytogenes was assumed to be 6 log CFU/mL in raw milk. The inclusion of inhibitory LAB also reduced risk of listeriosis due to soft cheese consumption, but not as much as for semi-hard cheese. QMRA results predicted that consumption of contaminated cheeses can carry a high risk of listeriosis, especially for vulnerable populations. Scenario analyses indicated that aging of semi-hard cheese and inclusion of antimicrobial LAB mix in semi-hard and soft cheeses are effective risk mitigation measures. | |
| dc.description.sponsorship | The authors thank to Fundação de Amparo à Pesquisa do Estado de São Paulo for the financial support (Grants 2014/14891-7 and 2016/ 09346-5) and to Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant # 302763/2014-7 and #305804/2017-0), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Grant #33003017027P1). Dr. Gonzales-Barron wishes to acknowledge the financial support provided by the Portuguese Foundation for Science and Technology (FCT) through the award of a five-year Investigator Fellowship (IF) in the mode of Development Grants (IF/00570). | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | en_EN |
| dc.identifier.citation | Campagnollo, Fernanda Bovo; Gonzales-Barron, Ursula; Pilão Cadavez, Vasco Augusto; Sant'Ana, Anderson S.; Schaffner, Donald W. (2018). Quantitative risk assessment of Listeria monocytogenes in traditional Minas cheeses: the cases of artisanal semi-hard and fresh soft cheeses. Food Control. ISSN 0956-7135. 92, p. 370-379 | en_EN |
| dc.identifier.doi | 10.1016/j.foodcont.2018.05.019 | en_EN |
| dc.identifier.issn | 0956-7135 | |
| dc.identifier.uri | http://hdl.handle.net/10198/14485 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | en_EN |
| dc.subject | Dairy products | en_EN |
| dc.subject | Food inspection | en_EN |
| dc.subject | Food safety | en_EN |
| dc.subject | Intervention strategies | en_EN |
| dc.subject | Natural antimicrobial | en_EN |
| dc.subject | Traditional foods | en_EN |
| dc.title | Quantitative risk assessment of Listeria monocytogenes in traditional Minas cheeses: the cases of artisanal semi-hard and fresh soft cheeses | en_EN |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Gonzales-Barron | |
| person.familyName | Cadavez | |
| person.givenName | Ursula | |
| person.givenName | Vasco | |
| person.identifier | R-000-HDG | |
| person.identifier.ciencia-id | 0813-C319-B62A | |
| person.identifier.ciencia-id | 441B-01AB-A12E | |
| person.identifier.orcid | 0000-0002-8462-9775 | |
| person.identifier.orcid | 0000-0002-3077-7414 | |
| person.identifier.rid | A-3958-2010 | |
| person.identifier.scopus-author-id | 9435483700 | |
| person.identifier.scopus-author-id | 9039121900 | |
| rcaap.rights | openAccess | en_EN |
| rcaap.type | article | en_EN |
| relation.isAuthorOfPublication | 17c6b98f-4fb5-41d3-839a-6f77ec70021a | |
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