Publication
Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase
| dc.contributor.author | Guimarães, Rafaela | |
| dc.contributor.author | Calhelha, Ricardo C. | |
| dc.contributor.author | Froufe, Hugo J.C. | |
| dc.contributor.author | Abreu, Rui M.V. | |
| dc.contributor.author | Carvalho, Ana Maria | |
| dc.contributor.author | Queiroz, Maria João R.P. | |
| dc.contributor.author | Ferreira, Isabel C.F.R. | |
| dc.date.accessioned | 2016-09-29T15:01:11Z | |
| dc.date.available | 2016-09-29T15:01:11Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Angiogenesis is a process by which new blood vessels are formed from the pre-existing vasculature, and it is a key process that leads to tumour development. Some studies have recognized phenolic compounds as chemopreventive agents; flavonoids, in particular, seem to suppress the growth of tumor cells modifying the cell cycle. Herein, the antiangiogenic activity of Roman chamomile (Chamaemelum nobile L.) extracts (methanolic extract and infusion) and the main phenolic compounds present (apigenin, apigenin-7-O-glucoside, caffeic acid, chlorogenic acid, luteolin, and luteolin-7-O-glucoside) was evaluated through enzymatic assays using the tyrosine kinase intracellular domain of the Vascular Endothelium Growth Factor Receptor-2 (VEGFR-2), which is a transmembrane receptor expressed fundamentally in endothelial cells involved in angiogenesis, and molecular modelling studies. The methanolic extract showed a lower IC50 value (concentration that provided 50% of VEGFR-2 inhibition) than the infusion, 269 and 301 μg mL(-1), respectively. Regarding phenolic compounds, luteolin and apigenin showed the highest capacity to inhibit the phosphorylation of VEGFR-2, leading us to believe that these compounds are involved in the activity revealed by the methanolic extract. | pt_PT |
| dc.description.sponsorship | The authors are grateful to strategic projects PEst-OE/AGR/UI0690/2014 and PEst-C/QUI/UI0686/2013-2014 for financial support to the research centres. R. Guimarães, and R. Calhelha thank to FCT, POPH-QREN and FSE for their grants (SFRH/BD/78307/2011 and SFRH/BPD/68344/2010). | pt_PT |
| dc.identifier.citation | Guimarães, Rafaela; Calhelha, Ricardo C.; Froufe, Hugo J.C.; Abreu, Rui M.V.; Carvalho, Ana Maria; Queiroz, Maria João R.P.; Ferreira, Isabel C.F.R. (2016). Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase. Food & Function. ISSN 2042-6496. 7:1, p. 79-83 | pt_PT |
| dc.identifier.doi | 10.1039/c5fo00586h | pt_PT |
| dc.identifier.issn | 2042-6496 | |
| dc.identifier.uri | http://hdl.handle.net/10198/13341 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Royal Society of Chemistry | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Wild Roman chamomile extracts and phenolic compounds: enzymatic assays and molecular modelling studies with VEGFR-2 tyrosine kinase | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FQUI%2FUI0686%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/Incentivo%2FQUI%2FUI0686%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F78307%2F2011/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F68344%2F2010/PT | |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 79-83 | pt_PT |
| oaire.citation.title | Food & Function | pt_PT |
| oaire.citation.volume | 7 | pt_PT |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | COMPETE | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | SFRH | |
| oaire.fundingStream | SFRH | |
| person.familyName | Calhelha | |
| person.familyName | Abreu | |
| person.familyName | Carvalho | |
| person.familyName | Ferreira | |
| person.givenName | Ricardo C. | |
| person.givenName | Rui M.V. | |
| person.givenName | Ana Maria | |
| person.givenName | Isabel C.F.R. | |
| person.identifier | ID G-7399-2011 | |
| person.identifier | 144781 | |
| person.identifier.ciencia-id | F313-E3CE-554E | |
| person.identifier.ciencia-id | 0F19-0DE2-12A2 | |
| person.identifier.ciencia-id | D31A-35AF-E2A9 | |
| person.identifier.ciencia-id | 9418-CF95-9919 | |
| person.identifier.orcid | 0000-0002-6801-4578 | |
| person.identifier.orcid | 0000-0002-7745-8015 | |
| person.identifier.orcid | 0000-0001-5508-5935 | |
| person.identifier.orcid | 0000-0003-4910-4882 | |
| person.identifier.rid | J-2172-2014 | |
| person.identifier.rid | E-8500-2013 | |
| person.identifier.scopus-author-id | 6507978333 | |
| person.identifier.scopus-author-id | 7003290613 | |
| person.identifier.scopus-author-id | 20336503900 | |
| person.identifier.scopus-author-id | 36868826600 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 2d5d1a41-7561-4a01-871c-b4c97da35053 | |
| relation.isAuthorOfPublication | cadb03a4-5e60-4745-b35f-fc3a97c071bc | |
| relation.isAuthorOfPublication | 977f5254-f7c5-4c91-873f-2700ecd4692f | |
| relation.isAuthorOfPublication | bd0d1537-2e03-41fb-b27a-140af9c35db8 | |
| relation.isAuthorOfPublication.latestForDiscovery | 2d5d1a41-7561-4a01-871c-b4c97da35053 | |
| relation.isProjectOfPublication | f3fc3f7e-17b1-488f-abea-2601a44654b1 | |
| relation.isProjectOfPublication | f20e17dd-04b5-4a8e-b506-73cad03a100c | |
| relation.isProjectOfPublication | 3737f7a3-a26b-4aca-9fb3-5a453a4121f0 | |
| relation.isProjectOfPublication | 2e073d8e-a10e-4fde-8f3a-06fdc87acb03 | |
| relation.isProjectOfPublication | 11192b59-0b2d-42b9-bf36-31bc50f8bccc | |
| relation.isProjectOfPublication.latestForDiscovery | 2e073d8e-a10e-4fde-8f3a-06fdc87acb03 |