Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC-ELSD

Lama, Aelina; Silvério, Sara; Veloso, Ana C.A.; Rodrigues, Lígia R.; Dias, Teresa; Peres, António M.

Publication

Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC-ELSD

2017Conference object

dc.contributor.author	Lama, Aelina
dc.contributor.author	Silvério, Sara
dc.contributor.author	Veloso, Ana C.A.
dc.contributor.author	Rodrigues, Lígia R.
dc.contributor.author	Dias, Teresa
dc.contributor.author	Peres, António M.
dc.date.accessioned	2020-02-28T11:56:21Z
dc.date.available	2020-02-28T11:56:21Z
dc.date.issued	2017
dc.description.abstract	Fructooligosaccharides (FOS) are present in plants and fruits at low concentrations, thus their extraction from natural sources may not be economically viable for a large scale industrial application. Therefore, FOS production by fermentation using fungi can be an alternative. In this work, FOS were produced using Aspergillus aculeatus at different sucrose initial concentrations (88 to 265 g/L) and at temperatures from 22 to 32ºC. FOS production was monitored by HPLC-ELSD, allowing to confirm that the initial sucrose concentration significantly influenced biomass growth (a maximum value of 16 ± 2 g was achieved) although it did not significantly affect the maximum FOS yield (amount of FOS produced per initial sucrose) obtained, which varied from 51 to 59 g/g) obtained, which varied from 51 to 59 g/g. Finally, the preliminary results enabled verifying that depending on the fermentation conditions, slightly different FOS production profiles were obtained (Figure 1), revealing differences in the individual FOS concentrations (i.e., 1-kestose, nystose and fructofuranosylnystose), which could be of interest since it has been reported that the beneficial health effects of FOS may depend on the relative FOS composition.	pt_PT
dc.description.sponsorship	This work was also financially supported by Project POCI-01–0145-FEDER-006984–Associate Laboratory LSRE-LCM, UID/AGR/00690/2013 – CIMO and UID/BIO/04469/2013 funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia, Portugal.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Lama, Aelina; Silvério, Sara; Veloso, Ana C.A.; Rodrigues, Lígia R.; Dias, Teresa; Peres, António M. (2017). Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC ELSD. In Peres, António M.; Barros, Lillian; Dias, L.G.; Ferreira, Isabel C.F.R. (Eds) 10º Encontro Nacional de Cromatografia: livro de resumos. Bragança: Instituto Politécnico. ISBN 978-972-745-234-7	pt_PT
dc.identifier.isbn	978-972-745-234-7
dc.identifier.uri	http://hdl.handle.net/10198/20756
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Instituto Politécnico de Bragança	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.title	Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC-ELSD	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.awardURI	info:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT
oaire.citation.conferencePlace	Bragança, Portugal	pt_PT
oaire.citation.title	10º Encontro de Cromatografia	pt_PT
oaire.fundingStream	5876
oaire.fundingStream	5876
person.familyName	Dias
person.familyName	Peres
person.givenName	Teresa
person.givenName	António M.
person.identifier	142703
person.identifier	107333
person.identifier.ciencia-id	CF16-5443-F420
person.identifier.orcid	0000-0002-9419-9561
person.identifier.orcid	0000-0001-6595-9165
person.identifier.rid	I-8470-2012
person.identifier.scopus-author-id	56830642600
person.identifier.scopus-author-id	7102331969
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT
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