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Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC-ELSD

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dc.contributor.authorLama, Aelina
dc.contributor.authorSilvério, Sara
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorRodrigues, Lígia R.
dc.contributor.authorDias, Teresa
dc.contributor.authorPeres, António M.
dc.date.accessioned2020-02-28T11:56:21Z
dc.date.available2020-02-28T11:56:21Z
dc.date.issued2017
dc.description.abstractFructooligosaccharides (FOS) are present in plants and fruits at low concentrations, thus their extraction from natural sources may not be economically viable for a large scale industrial application. Therefore, FOS production by fermentation using fungi can be an alternative. In this work, FOS were produced using Aspergillus aculeatus at different sucrose initial concentrations (88 to 265 g/L) and at temperatures from 22 to 32ºC. FOS production was monitored by HPLC-ELSD, allowing to confirm that the initial sucrose concentration significantly influenced biomass growth (a maximum value of 16 ± 2 g was achieved) although it did not significantly affect the maximum FOS yield (amount of FOS produced per initial sucrose) obtained, which varied from 51 to 59 g/g) obtained, which varied from 51 to 59 g/g. Finally, the preliminary results enabled verifying that depending on the fermentation conditions, slightly different FOS production profiles were obtained (Figure 1), revealing differences in the individual FOS concentrations (i.e., 1-kestose, nystose and fructofuranosylnystose), which could be of interest since it has been reported that the beneficial health effects of FOS may depend on the relative FOS composition.pt_PT
dc.description.sponsorshipThis work was also financially supported by Project POCI-01–0145-FEDER-006984–Associate Laboratory LSRE-LCM, UID/AGR/00690/2013 – CIMO and UID/BIO/04469/2013 funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLama, Aelina; Silvério, Sara; Veloso, Ana C.A.; Rodrigues, Lígia R.; Dias, Teresa; Peres, António M. (2017). Monitoring fructooligossacharides production using Aspergillus aculeatus by HPLC ELSD. In Peres, António M.; Barros, Lillian; Dias, L.G.; Ferreira, Isabel C.F.R. (Eds) 10º Encontro Nacional de Cromatografia: livro de resumos. Bragança: Instituto Politécnico. ISBN 978-972-745-234-7pt_PT
dc.identifier.isbn978-972-745-234-7
dc.identifier.urihttp://hdl.handle.net/10198/20756
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Bragançapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.titleMonitoring fructooligossacharides production using Aspergillus aculeatus by HPLC-ELSDpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04469%2F2013/PT
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.title10º Encontro de Cromatografiapt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameDias
person.familyNamePeres
person.givenNameTeresa
person.givenNameAntónio M.
person.identifier142703
person.identifier107333
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id7102331969
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication87c9cae0-314d-4622-ba85-4d3ec5726606
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery87c9cae0-314d-4622-ba85-4d3ec5726606
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relation.isProjectOfPublication774297b8-449c-4471-8bbb-0cfe1f4c8275
relation.isProjectOfPublication.latestForDiscovery774297b8-449c-4471-8bbb-0cfe1f4c8275

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