Publication
Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi
| dc.contributor.author | Martins, Ivone | |
| dc.contributor.author | Meirinho, Sofia G. | |
| dc.contributor.author | Dias, Teresa | |
| dc.contributor.author | Jorge, Lurdes | |
| dc.contributor.author | Martins, Fátima | |
| dc.contributor.author | Choupina, Altino | |
| dc.date.accessioned | 2011-02-14T10:22:05Z | |
| dc.date.available | 2011-02-14T10:22:05Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Phytophthora cinnamomi is a soil-borne pseudofungus belonging to the Class Oomycetes or „water moulds' in the Kingdom Chromista (Figure 1). Is one among the most destructive species of Phytophthora associated to the decline of forestry, ornamental and fruit species, as well as of some 900 other woody perennial plant species Associated with this oomycete is the ink disease of Castanea Sativa Mill. Glucan endo-1,3-β-D-glucosidase (EC 3.2.1.39) catalyzes de hydrolysis of 1,3-β-D-glucoside linkages in callose, laminarin and several carbohydrates found in the cell wall of plants and fungi. It is generally thought that glucanases play a role in plant defense by digesting wall components of the fungal pathogen. In yeast, 1,3-- glucanases have been studied for their role in germination, sporulation, mating and cell growth since they are regulated in cell cycle dependent manner, and are differentially expressed during vegetative growth, mating and the late stages of sporulating diploid. In plant, 1,3--glucanases have been characterized for their major role in plant defence, as well as for their involvement in germination, microsporogenesis and embryogenesis. In oomycetes, glucanases have been studied on a biochemical level for their possible role in hyphal tip growth and branching where there is thought to be a delicate balance between cell wall synthesis and hydrolyses. In the present work, we obtained a fragment with 1231bp of the endo-1,3--glucanase gene by standard PCR, using conserved primers and the whole genomic sequence with 2586 bp was obtained by amplifying the previous sequence by asymmetric PCR. The gene expression was studied during growth in different carbon sources and was also performed a time course of endo-1,3-β-D-glucosidase production. | |
| dc.identifier.citation | Martins, Ivone; Meirinho, Sofia; Dias, Teresa; Jorge, Lurdes; Martins, Fátima; Choupina, Altino (2010). Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi. In 9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologia. Évora | por |
| dc.identifier.uri | http://hdl.handle.net/10198/3371 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Sociedade Portuguesa de Fitopatologia | por |
| dc.title | Cloning and expression analysis of glucanase genes from Phytophthora cinnamomi | por |
| dc.type | conference poster | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67628%2F2006/PT | |
| oaire.citation.conferencePlace | Évora | por |
| oaire.citation.title | 9th Conference of the European Foundation for Plant Pathology. 6th Congress of the Sociedade Portuguesa de Fitopatologia. | por |
| oaire.fundingStream | 3599-PPCDT | |
| person.familyName | Dias | |
| person.familyName | Choupina | |
| person.givenName | Teresa | |
| person.givenName | Altino | |
| person.identifier | 142703 | |
| person.identifier | 587972 | |
| person.identifier.ciencia-id | 1A14-77FC-9656 | |
| person.identifier.orcid | 0000-0002-9419-9561 | |
| person.identifier.orcid | 0000-0002-3956-9398 | |
| person.identifier.rid | L-5382-2014 | |
| person.identifier.scopus-author-id | 56830642600 | |
| person.identifier.scopus-author-id | 14051602500 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |
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