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Chemical profile and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of geopropolis from the stingless bee Melipona orbignyi

2017Journal article Open access
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dc.contributor.authorSantos, H.S.
dc.contributor.authorCampos, Jaqueline Ferreira
dc.contributor.authorSantos, Cintia Miranda
dc.contributor.authorBalestieri, José Benedito Perrella
dc.contributor.authorSilva, Denise Brentan
dc.contributor.authorCarollo, Carlos Alexandre
dc.contributor.authorSouza, Kely de Picoli
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorSantos, Edson Lucas dos
dc.date.accessioned2018-01-31T10:00:00Z
dc.date.accessioned2018-01-31T15:40:27Z
dc.date.available2018-01-31T10:00:00Z
dc.date.available2018-01-31T15:40:27Z
dc.date.issued2017
dc.description.abstractLicensee MDPI, Basel, Switzerland. Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the Melipona orbignyi geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in Saccharomyces cerevisiae colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in S. cerevisiae. In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of M. orbignyi and demonstrates its pharmacological properties.en_EN
dc.description.sponsorshipThis work was supported by grants from Foundation to Support to Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT, Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and PRODER, (24.073 – Â, Portugal).
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSantos, Helder Freitas; Campos, Jaqueline Ferreira; Santos, Cintia Miranda; Balestieri, José Benedito Perrella; Silva, Denise Brentan; Carollo, Carlos Alexandre; Souza, Kely de Picoli; Estevinho, Leticia M.; Santos, Edson Lucas (2017). Chemical profile and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of geopropolis from the stingless bee Melipona orbignyi. International Journal of Molecular Sciences. ISSN 1661-6596. 18en_EN
dc.identifier.doi10.3390/ijms18050953en_EN
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10198/15355
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectHPLC-DAD-MS 1en_EN
dc.subjectHyaluronidase 4en_EN
dc.subjectLipid peroxidation 3en_EN
dc.subjectMicroorganisms 6en_EN
dc.subjectMutation 5en_EN
dc.subjectPhenolic compounds 2en_EN
dc.titleChemical profile and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of geopropolis from the stingless bee Melipona orbignyien_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameEstevinho
person.givenNameLetícia M.
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.orcid0000-0002-9249-1948
person.identifier.scopus-author-id6506577664
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublication.latestForDiscovery4a1d5ba2-1854-4ca5-89a4-73f35e964df9

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