Orientador(es)
Resumo(s)
In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of
Brassica oleracea var. costata and its antioxidant capacity. Samples were obtained in different culture
medium, with distinct supplementations to verify their influence on those parameters. Phenolic
determination was achieved by HPLC-DAD. Antioxidant activity was assessed against DPPH•. In
calli and roots no phenolic compound was identified. In shoots was verified the presence of 36
compounds, which included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives),
and hydroxycinnamic acyl glycosides (with a predominance of synapoyl gentiobiosides). MS liquid
medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid
(NAA) revealed to be the best in vitro condition to produce shoot material with highest phenolic
compound contents and stronger antioxidant potential, thus with a possible increase of health benefits.
Descrição
Palavras-chave
Brassica oleracea L. var. costata DC Shoots Calli Roots Phenolic compounds Antioxidant activity
Contexto Educativo
Citação
Taveira, M.; Pereira, D.M.; Sousa, C.; Ferreres, F.; Andrade, P.B.; Martins, A.; Pereira, J.A.; Valentão, P. (2009). In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant phenolic compounds. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 57:4, p. 1247-1252