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Chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in Northeast Portugal

2016Journal article Open access
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dc.contributor.authorSobral, Filipa
dc.contributor.authorSampaio, Andreia
dc.contributor.authorFalcão, Soraia
dc.contributor.authorQueiroz, Maria João R.P.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorVilas-Boas, Miguel
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2016-11-14T16:01:55Z
dc.date.available2016-11-14T16:01:55Z
dc.date.issued2016
dc.description.abstractBee venom (BV) or apitoxin is a complex mixture of substances with reported biological activity. In the present work, five bee venom samples obtained from Apis mellifera iberiensis from the Northeast Portugal (two different apiaries) were chemically characterized and evaluated for their antioxidant, anti-inflammatory and cytotoxic properties. The LC/DAD/ESI-MS(n) analysis of the samples showed that melittin was the most abundant compound, followed by phospholipase A2 and apamin. All the samples revealed antioxidant and anti-inflammatory activity but without a direct relation with any of the individual chemical components identified. The results highlight that there are specific concentrations (present in BV5) in which these compounds are more active. The BV samples showed similar cytotoxicity for all the tested tumour cell lines (MCF-7, NCI-H460, HeLa and HepG2), being MCF-7 and HeLa the most susceptible ones. Nevertheless, the studied samples seem to be suitable to treat breast, hepatocellular and cervical carcinoma because at the active concentrations, the samples were not toxic for non-tumour cells (PLP2). Regarding the non-small cell lung carcinoma, BV should be used under the toxic concentration for non-tumour cells. Overall, the present study corroborates the enormous bioactive potential of BV being the first report on samples from Portugal.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (PEst-OE/AGR/UI0690/2014) and R.C. Calhelha grant (SFRH/BPD/68344/2010).pt_PT
dc.identifier.citationSobral, Filipa; Sampaio, Andreia; Falcão, Soraia; Queiroz, Maria João R.P.; Calhelha, Ricardo C.; Vilas-Boas, Miguel; Ferreira, Isabel C.F.R. (2016). Chemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in Northeast Portugal. Food and Chemical Toxicology. ISSN 0278-6915. 94, p. 172–177pt_PT
dc.identifier.doi10.1016/j.fct.2016.06.008pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10198/13492
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBee venompt_PT
dc.subjectApaminpt_PT
dc.subjectMelittinpt_PT
dc.subjectPhospholipase A2pt_PT
dc.subjectAntioxidant/anti-inflammatorypt_PT
dc.subjectCytotoxicpt_PT
dc.titleChemical characterization, antioxidant, anti-inflammatory and cytotoxic properties of bee venom collected in Northeast Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0690%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F68344%2F2010/PT
oaire.citation.endPage177pt_PT
oaire.citation.startPage172pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume94pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameFalcão
person.familyNameCalhelha
person.familyNameVilas-Boas
person.familyNameFerreira
person.givenNameSoraia
person.givenNameRicardo C.
person.givenNameMiguel
person.givenNameIsabel C.F.R.
person.identifier711432
person.identifier1693134
person.identifier144781
person.identifier.ciencia-idD41A-6BED-4CBB
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-idA918-C6FF-81A4
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0003-3735-6951
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0002-8665-5280
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridI-5949-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id14832459200
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id6602648497
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5746ab3e-8ffe-4e26-9a41-8392cf031f28
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