Repository logo
 
Publication

Phenolic profiles of horse chestnut seed extracts obtained by ultrasound-assisted extraction

2021Conference object Open access
Simple item page
dc.contributor.authorAlbiston, Carly
dc.contributor.authorDias, Maria Inês
dc.contributor.authorPinela, José
dc.contributor.authorAñibarro-Ortega, Mikel
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2022-01-21T15:51:14Z
dc.date.available2022-01-21T15:51:14Z
dc.date.issued2021
dc.description.abstractThe horse chestnut (Aesculus hippocastanum L.) is a deciduous tree widely cultivated in urban areas with temperate climate across the Northern hemisphere [1]. Among the scientific community, this species is known mainly for the steroidal glycosides or polycyclic terpenes resulting from the secondary metabolism, among which aescin is the main active component and responsible for most of its medicinal properties [2,3]. While the bark has been used to treat dermatitis, the leaves and seeds are used due to their anti-inflammatory effects [3]. In fact, horse chestnut seed is an important raw material in the pharmaceutical industry. On the other hand, bioactive phenolic compounds such as quercetin and kaempferol glycosides can also be found in this raw material [4]. Therefore, this study was carried out to characterize the phenolic composition of the horse chestnut seed kernel, shell, and pellicle. The different parts of the seed were hand-separated with a knife, lyophilized, and ground to a fine powder. Hydroethanolic extracts were then obtained by ultrasound-assisted extraction, using 20% ethanol and 40 min sonication at 400 W (at 20 kHz frequency). The phenolic profiles were characterized by high- performance liquid chromatography coupled with diode array detector and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn). Quantification was made based on calibration curves contracted with commercial standards of the most similar phenolic compounds. The horse chestnut seed kernel was particularly rich in flavonoids, among which kaempferol-O- pentoside-O-hexoside-O-hexoside and isorhamnetin-O-pentoside-O-di-hexoside were predominant compounds. In turn, the seed pellicle extract contained mostly flavan-3-ols (β-type (epi)catechin derivatives). The shell also contained flavan-3-ols and flavonoids, where (-)- epicatechin and kaempferol-O-deoxyhexoside-O-deoxyhexoside were the phenolic compounds with the highest abundance in each group, respectively. In general, the pellicle was the seed part with the highest content of phenolic compounds, followed by the kernel. Thus, this study showed that the different parts of the inedible horse chestnut seeds have different phenolic profiles in qualitative and quantitative terms. Further analyses to assess the in vitro antioxidant, anti- inflammatory, antimicrobial, and cytotoxic activities of the obtained seed extracts are underway.pt_PT
dc.description.sponsorshipTo the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020); for the M. Añibarro-Ortega PhD grant (2020.06297.BD), the contracts of M.I. Dias and L. Barros through the institutional scientific employment program-contract, and the contract of J. Pinela (CEECIND/01011/2018) through the individual scientific employment program-contract; to the C. Albiston grant through CIMO funds (Project PlantCovid, NORTE-01-02B7-FEDER-054870); and to the FEDER-Interreg España- Portugal programme for financial support through the Project TRANSCoLAB 0612_TRANS_CO_LAB_2_P and to the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project GreenHealth - Norte-01-0145-FEDER-000042.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlbiston, Carly; Dias, Maria Inês; Pinela, José; Añibarro-Ortega, Mikel; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Phenolic profiles of horse chestnut seed extracts obtained by ultrasound-assisted extraction. In 7th Portuguese Young Chemists Meeting. ISBN 978-989-8124-31-0pt_PT
dc.identifier.isbn978-989-8124-31-0
dc.identifier.urihttp://hdl.handle.net/10198/24849
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAesculus hippocastanum L.pt_PT
dc.titlePhenolic profiles of horse chestnut seed extracts obtained by ultrasound-assisted extractionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F01011%2F2018%2FCP1578%2FCT0002/PT
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.title7th Portuguese Young Chemists Meetingpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
person.familyNameDias
person.familyNamePinela
person.familyNameAñibarro-Ortega
person.familyNameFerreira
person.familyNameBarros
person.givenNameMaria Inês
person.givenNameJosé
person.givenNameMikel
person.givenNameIsabel C.F.R.
person.givenNameLillian
person.identifier144781
person.identifier469085
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-idC61D-DD88-73C7
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-2835-5079
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridB-4466-2014
person.identifier.ridE-8500-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublication706cd9ff-065b-4c98-98a7-5c0a9e45adc4
relation.isAuthorOfPublication7235cb7a-09a1-453b-b39b-52989046fab6
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublication.latestForDiscovery7235cb7a-09a1-453b-b39b-52989046fab6
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublicationec142c57-5163-4512-9a14-7319866da393
relation.isProjectOfPublication.latestForDiscoveryec142c57-5163-4512-9a14-7319866da393

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
8. 7PYCheM (May 2021) Portugal - Poster internacional - Albiston et al 2021.pdf
Size:
975.87 KB
Format:
Adobe Portable Document Format
Download

Collections

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus