Publication
Electrochemical aptasensor array for multiple detection of human osteopontin
| dc.contributor.author | Meirinho, Sofia G. | |
| dc.contributor.author | Dias, L.G. | |
| dc.contributor.author | Peres, António M. | |
| dc.contributor.author | Rodrigues, Lígia R. | |
| dc.date.accessioned | 2018-02-20T16:48:48Z | |
| dc.date.available | 2018-02-20T16:48:48Z | |
| dc.date.issued | 2016 | |
| dc.description.abstract | Most cancer diseases are associated with the presence of several biomarkers, as the proteins. The proteins, when overexpressed in biological fluids could be used as poten:al diagnos:c or prognos:c markers indica:ve of disease states [1,2]. Osteopon:n (OPN) is a phosphorylated glycoprotein and has been reported as a poten:al breast cancer biomarker, its overexpression may be indica:ve of tumor forma:on, cancer progression, metastasis and poor prognosis [3–6]. An early diagnosis of the disease is of utmost importance to improve the pa:ent’s survival rate and therapeu:c efficacy, as well as to monitor the disease recurrence. In this sense, it is essen:al to develop new, simple and cost-effec:ve methods holding a high sensi:vity and reliability for the simultaneous detec:on of mul:ple protein disease biomarkers in biological fluids [7]. The electrochemical mul:-aptasensors arrays has recently a\racted a\en:on for the detec:on of various proteins disease biomarkers, due to their advantages, such as high sensi:vity, specificity, fast detec:on, easier experimental procedures, lower cost prepara:on and the poten:al for miniaturiza:on, as well as advantages of aptamers as bioreceptors elements such as high affinity and specificity towards a specific target, easy to produce and synthesize, good stability and amenable to chemical modifica:on [8– 10]. This study describes the development of an electrochemical mul:-aptasensor array for the simultaneous detec:on of human OPN using two specific aptamers. To enable mul:plexed protein assay, the RNA and DNA aptamers were immobilized in the working electrodes of the dual-screen-printed gold working electrodes (dual-SPGEs) via streptavidin-bio:n interac:on and using the [Fe(CN)6]3-/4- as the redox probe for cyclic voltammetry (CV) measurements. The preliminary results herein report showed a good response of the mul:-aptasensor array for the detec:on of human OPN. Moreover, the DNA/RNA mul:- aptasensor array was able to selec:vely detect human OPN in the presence of other interfering proteins such as thrombin, bovine serum albumin, bovine osteopon:n and lysozyme. Considering these preliminary results, the mul:-aptasensor array holding the two aptamers could be a good alterna:ve for the specific detec:on of human OPN and for cancer diagnosis overall. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Meirinho, Sofia G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2018). Electrochemical aptasensor array for multiple detection of human osteopontin. In XXI Meeting of the Portuguese Electrochemistry Society & XVIII Iberian Electrochemistry Meeting: abstract book. Bragança: Instituto Politécnico. ISBN 978-972-745-213-2 | pt_PT |
| dc.identifier.isbn | 978-972-745-213-2 | |
| dc.identifier.uri | http://hdl.handle.net/10198/15852 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Instituto Politécnico de Bragança | pt_PT |
| dc.relation.publisherversion | http://hdl.handle.net/10198/12931 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Electrochemical aptasensor array for multiple detection of human osteopontin | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Bragança, Portugal | pt_PT |
| oaire.citation.endPage | 24 | pt_PT |
| oaire.citation.startPage | 24 | pt_PT |
| oaire.citation.title | Abstract Book - XXI Meeting of the Portuguese Electrochemistry Society & XVIII Iberian Electrochemistry Meeting | pt_PT |
| person.familyName | Dias | |
| person.familyName | Peres | |
| person.givenName | Luís G. | |
| person.givenName | António M. | |
| person.identifier | 107333 | |
| person.identifier.ciencia-id | 2F11-9092-FAAF | |
| person.identifier.ciencia-id | CF16-5443-F420 | |
| person.identifier.orcid | 0000-0002-1210-4259 | |
| person.identifier.orcid | 0000-0001-6595-9165 | |
| person.identifier.rid | I-8470-2012 | |
| person.identifier.scopus-author-id | 23569169900 | |
| person.identifier.scopus-author-id | 7102331969 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | eac8c166-4056-4ed0-8d8d-7ecb2c4481a5 | |
| relation.isAuthorOfPublication | 7d93be47-8dc4-4413-9304-5b978773d3bb | |
| relation.isAuthorOfPublication.latestForDiscovery | 7d93be47-8dc4-4413-9304-5b978773d3bb |
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