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Antioxidant potential of fungi and seedlings during mycorrhizal induction

dc.contributor.authorReis, Filipa S.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorCarvalho, Filipa Arminda
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorMartins, Anabela
dc.date.accessioned2013-09-06T10:03:55Z
dc.date.available2013-09-06T10:03:55Z
dc.date.issued2013
dc.description.abstractMycorrhizal associations implicate a chemical recognizing process being the establishment of the symbiosis triggered by signals produced by both partners. We evaluated the establishment of mycorrhizal symbiosis between two ectomycorrhizal (ECM) fungi species, Paxillus involutus and Pisolithus arhizus, and two different symbionts, Pinus pinaster and Castanea sativa. ln a first step, we monitored the growth of the two ECM species in the presence and absence of the symbiont P. pinaster. Then we evaluated the antioxidant properties of fungi and seedlings, in response to the symbiotic process, under different contact periods. After, we tested the mycorrhization process between the two fungi species and C. sativa seedlings, assessing the production of tocopherols and sugars produced by both partners during the earlier steps of symbiosis. The antioxidant properties were assessed through four in vitro assays: Folin-Ciocalteu assay (total phenolics), Ferricyanide/Prussian blue assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and Beta-carotene/linoleate system assay. Tocopherols and sugars were analysed by HPLC-fluorescence and HPLC-RI, respectively. ECM fungi grew less in the presence of P. pinaster, with P. arhizus being less affected (in growth) and thus being more adapted to this association. Regarding the mechanisms of oxidative stress in ectomycorrhizal associations, t he response of the fungi and plants to the contact with the host is somehow specific, revealing different forms of mutual recognition. Considering bioactive compounds production, P. involutus revea led a maximal value of phenolics after 6h in co-culture with P. pinaster and P. arhizus revealed increased contents of sugars and tocopherols when in contact with C. sativa.por
dc.identifier.citationReis, Filipa S.; Carocho, Márcio; Carvalho, Filipa A.; Ferreira, Isabel C.F.R.; Martins, Anabela (2013). Antioxidant potential of fungi and seedlings during mycorrhizal induction. In 7th International Conference on Mycorrhiza. New Delhi, Indiapor
dc.identifier.urihttp://hdl.handle.net/10198/8650
dc.language.isoengpor
dc.peerreviewedyespor
dc.titleAntioxidant potential of fungi and seedlings during mycorrhizal inductionpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceNew Delhi, Indiapor
oaire.citation.title7th International Conference on Mycorrhiza (ICOM7), 6 a 11 de Janeiro de 2013por
person.familyNameReis
person.familyNameCarocho
person.familyNameFerreira
person.familyNameMartins
person.givenNameFilipa S.
person.givenNameMárcio
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifierawbWiiMAAAAJ&hl
person.identifier144781
person.identifier.ciencia-id391F-AFE1-64C7
person.identifier.ciencia-id5614-005B-5CF1
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-9159-0530
person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridI-2093-2013
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id36982144400
person.identifier.scopus-author-id52463229800
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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relation.isAuthorOfPublication.latestForDiscovery46b39e1f-2674-4be1-a96a-c2e5792e6979

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