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Targeting Skin Aging Hallmarks In Vitro: Antioxidant, Anti-Inflammatory, and Anti-Senescence Effects of Phenolic-Rich Extracts from Cistus L. Species

2026Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg04:Educação de Qualidade
dc.contributor.authorMarques, Mário Pedro
dc.contributor.authorLandim, Euclides
dc.contributor.authorVarela, Carla
dc.contributor.authorCosta, Ricardo M. F. da
dc.contributor.authorMarques, Joana
dc.contributor.authorCarvalho, Luís A. E. Batista de
dc.contributor.authorSilva, Ana
dc.contributor.authorCruz, Maria Teresa
dc.contributor.authorAndré, Rebeca
dc.contributor.authorRijo, Patrícia
dc.contributor.authorDias, Maria Inês
dc.contributor.authorCarvalho, Aida
dc.contributor.authorOliveira, Paulo J.
dc.contributor.authorCabral, Célia
dc.date.accessioned2026-02-16T15:40:38Z
dc.date.available2026-02-16T15:40:38Z
dc.date.issued2026
dc.description.abstractPlant-based extracts are rich sources of phenolic compounds, which may act as skin antiaging mediators. Herein, Cistus albidus L. (Ca), Cistus ladanifer L. subsp. ladanifer (Cl) and Cistus salviifolius L. (Cs) were selected to test whether their phytochemical profile and bioactive potential align to target human skin aging. Hydroethanolic extracts (HEs) were prepared and characterized using infrared vibrational spectroscopy (FTIR-ATR) and liquid chromatography-mass spectrometry (LC-MS). Non-toxic concentrations were screened, and cytoprotective and antioxidant effects were studied in tert-butyl hydroperoxide-stimulated normal human dermal fibroblasts (NHDFs). Lipopolysaccharide-stimulated RAW 264.7 macrophages were used to assess anti-inflammatory activity, the Organization for Economic Co-operation and Development (OECD) Test Guideline No. 439 was used to assess irritant effects, and the anti-senescence potential was assessed in etoposide-stimulated NHDFs. A series of enzymatic inhibition assays was performed. All extracts comprised ellagic acid derivatives, as well as myricetin and quercetin derivatives in Cs and Ca. The HE of Cs was also markedly composed of ligstroside. At non-toxic concentrations, cytoprotective effects were observed in NHDFs. However, only Cs and Cl exhibited significant antioxidant activity in these cells (p < 0.001 and p < 0.0001, respectively). In addition to that, Cl demonstrated highly significant anti-inflammatory (p < 0.0001) and anti-senescence (p < 0.0001) effects. Cs and Cl showed a remarkable potential to inhibit elastase; in addition, Cs also showed anti-hyaluronidase and anti-tyrosinase activities. Meaningfully, Cs and Cl extracts did not exhibit skin irritant effects. The unveiled potential of Cl in skin aging offset highlights the need to elucidate the detailed mechanisms of action, paving the way for the development of skin anti-aging formulations.eng
dc.description.sponsorshipThis work was co-funded by the EU Recovery and Resilience Facility and Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia, under projects, LA/P/0058/2020 (DOI: 10.54499/LA/P/0058/2020), UID/PRR/4539/2025 (DOI: 10.54499/UID/PRR/04539/2025) and UID/04539/2025 and by national funds through FCT/MCTES (PIDDAC): CIMO UID/00690/2025 (10.54499/UID/00690/2025) and UID/PRR/00690/2025 (10.54499/UID/PRR/00690/2025); SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020). It was also supported by FCT through the projects UID/04567/2025 (DOI: 10.54499/UID/04567/2025) (CBIOS) and UID/50006/2025 (DOI: 10.54499/UID/50006/2025) (LAQV/REQUIMTE). We also thank national funding by FCT, P.I., through the institutional and individual scientific employment program contract for M. I. Dias (CEECINSTLA/00032/2022/CP3032/CT0001), PhD grant for M. P. Marques (PRT/BD/153391/2021, DOI: 10.54499/PRT/BD/153391/2021) and PhD grant for E. Landim (PRT/BD/154364/2022, DOI: 10.54499/PRT/BD/154364/2022).
dc.identifier.citationMarques, Mario P.; Landim, Euclides; Varela, Carla; Da Costa, Ricardo M. F.; Marques, Joana; De Carvalho, Luis A. E. B.; Silva, Ana; Cruz, Maria T.; Andre, Rebeca; Rijo, Patricia; Dias, Maria Inês; Carvalho, Aida; Oliveira, Paulo J.; Cabral, Celia (2026).Targeting Skin Aging Hallmarks In Vitro: Antioxidant, Anti-Inflammatory, and Anti-Senescence Effects of Phenolic-Rich Extracts from Cistus L. Species. Antioxidants. ISSN 2076-3921. 15:1, p. 1-25
dc.identifier.doi10.3390/antiox15010149
dc.identifier.issn2076-3921
dc.identifier.urihttp://hdl.handle.net/10198/35762
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationResearch Center for Biosciences & Health Technologies
dc.relationSmall scale power generation Unit using Biomass gasification - SUBe
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.ispartofAntioxidants
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCistus spp.
dc.subjectPlant extract
dc.subjectPhenolic compounds
dc.subjectCytoprotective
dc.subjectCytotoxicity
dc.subjectAnti-inflammatory
dc.subjectAntioxidant
dc.subjectAnti-senescence
dc.subjectIrritant effects
dc.titleTargeting Skin Aging Hallmarks In Vitro: Antioxidant, Anti-Inflammatory, and Anti-Senescence Effects of Phenolic-Rich Extracts from Cistus L. Specieseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberLA/P/0007/2020
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oaire.awardNumberPCIF/GVB/0197/2017
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oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleResearch Center for Biosciences & Health Technologies
oaire.awardTitleSmall scale power generation Unit using Biomass gasification - SUBe
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04567%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PCIF%2FGVB%2F0197%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F50006%2F2019/PT
oaire.citation.endPage25
oaire.citation.issue1
oaire.citation.startPage1
oaire.citation.titleAntioxidants
oaire.citation.volume15
oaire.fundingStream6817 - DCRRNI ID
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oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDias
person.familyNameCarvalho
person.givenNameMaria Inês
person.givenNameAida
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