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High resolution melting analysis of a COI mini-barcode as a simple approach for the entomological authentication of honey

2021Conference object Open access
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dc.contributor.authorHonrado, Mónica
dc.contributor.authorPinto, M. Alice
dc.contributor.authorLopes, Ana
dc.contributor.authorHenriques, Dora
dc.contributor.authorQuaresma, Andreia
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2022-10-25T11:30:13Z
dc.date.available2022-10-25T11:30:13Z
dc.date.issued2021
dc.description.abstractHoney is highly valued for its taste, aroma, content in bioactive compounds and for being a natural food. In the European Union (EU), market demand for honey is higher than the domestic production and therefore a substantial amount of honey is imported. According to a 2014 European Parliament report on fraud in the food chain, honey was ranked as the 6th food product most prone to adulterateration.1 Up until now, honey authenticity addressed mainly sugars addition and botanical origin. However, an increased attention has recently been paid to honey entomological origin as it also relates to its geographical origin since honeybees carrying mitochondrial DNA (mtDNA) of distinct ancestries can be found across Europe. While in Portugal the predominant mtDNA of the autochthonous subspecies Apis mellifera iberiensis belongs to the A-lineage, when moving towards the northeastern part of the Iberian Peninsula this lineage is gradually replaced by the M-lineage. The native distribution of the M-lineage A. m. mellifera expands from the Pyrenees to Scandinavia and from the British Isles to the Ural Mountains while the C-lineage A. m. ligustica and A. m. carnica are naturally found in the Apennine and Balkan peninsulas, respectivelypt_PT
dc.description.sponsorshipThis work was funded by the project-2022, financed by IFAP. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020), to Fenapícola and Capemel for supplying the Portuguese honeys, to Dr. Antonio Nanetti (CREA-AA) for the Italian honeys and to António Pajuelo (Pajuelo Consultores Apícolas S.L.) for the Spanish honeys. D. Henriques is supported by the project BeeHappy (POCI-01-0145-FEDER-029871) funded by FEDER, COMPETE 2020-POCI and FCT. A. R. Lopes and A. Quaresma acknowledge the PhD scholarship funded by the FCT (SFRH/BD/143627/2019 and DFA/BD/5155/2020, respectively).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHonrado, Mónica; Pinto, M. Alice; Lopes, Ana; Henriques, Dora; Quaresma, Andreia; Amaral, Joana S. (2021). High resolution melting analysis of a COI mini-barcode as a simple approach for the entomological authentication of honey. In XV Encontro de Química dos Alimentos. Funchalpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/26049
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEuropean Chemical Societypt_PT
dc.relationPOCI-01-0145-FEDER-029871pt_PT
dc.relationMountain Research Center
dc.relationDFA/BD/5155/2020pt_PT
dc.relationHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHigh resolution melting analysispt_PT
dc.subjectCOIpt_PT
dc.subjectEntomological authenticationpt_PT
dc.subjectHoneypt_PT
dc.titleHigh resolution melting analysis of a COI mini-barcode as a simple approach for the entomological authentication of honeypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleHoneybees Apis mellifera L. in the Azores: developing innovative molecular tools and uncovering host-pathogen interactions in a pre- and post-Varroa destructor invasion
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F143627%2F2019/PT
oaire.citation.conferencePlaceFunchalpt_PT
oaire.citation.endPage520pt_PT
oaire.citation.startPage519pt_PT
oaire.citation.titleXV Encontro de Química dos Alimentospt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameHonrado
person.familyNamePinto
person.familyNameLopes
person.familyNameHenriques
person.familyNameQuaresma
person.familyNameAmaral
person.givenNameMónica
person.givenNameM. Alice
person.givenNameAna
person.givenNameDora
person.givenNameAndreia
person.givenNameJoana S.
person.identifier.ciencia-id4712-B40B-4B0E
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-id4F1A-4E4A-3F23
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-5126-4693
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0002-8678-5800
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id57119742600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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