Publicação
Development of an electrochemical aptasensor for the detection of human osteopontin
| dc.contributor.author | Meirinho, Sofia G. | |
| dc.contributor.author | Dias, L.G. | |
| dc.contributor.author | Peres, António M. | |
| dc.contributor.author | Rodrigues, Lígia R. | |
| dc.date.accessioned | 2015-05-11T14:13:19Z | |
| dc.date.available | 2015-05-11T14:13:19Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Electrochemical aptasensors, an emerging technology, enables the detection of protein biomarkers, which may be indicative of tumour activity. Osteopontin is a protein present in body fluids, being a possible biomarker since its overexpression has been related with breast cancer progression. An RNA aptamer, described in the literature, with affinity for human osteopontin, was synthetized, immobilized in a microelectrode gold surface and used for development electrochemical aptasensor for human OPN detection in standard solutions. Cyclic voltammetry results showed that this aptasensor allowed detecting human osteopontin with a detection limit of 8 nM, showing a satisfactory selectivity towards the target in the presence of others proteins, except for thrombin. | por |
| dc.identifier.citation | Meirinho, S.G.; Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. (2014). Development of an electrochemical aptasensor for the detection of human osteopontin. In XXVIII EUROSENSORS e Procedia Engineering. 87, p. 316-319 | por |
| dc.identifier.doi | 10.1016/j.proeng.2014.11.671 | |
| dc.identifier.uri | http://hdl.handle.net/10198/11810 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.title | Development of an electrochemical aptasensor for the detection of human osteopontin | por |
| dc.type | conference paper | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | EUROSENSORS 2014, Brescia, Itália | por |
| oaire.citation.endPage | 319 | por |
| oaire.citation.startPage | 316 | por |
| oaire.citation.title | Procedia Engineering | por |
| oaire.citation.volume | 87 | por |
| person.familyName | Dias | |
| person.familyName | Peres | |
| person.givenName | Luís G. | |
| person.givenName | António M. | |
| person.identifier | 107333 | |
| person.identifier.ciencia-id | 2F11-9092-FAAF | |
| person.identifier.ciencia-id | CF16-5443-F420 | |
| person.identifier.orcid | 0000-0002-1210-4259 | |
| person.identifier.orcid | 0000-0001-6595-9165 | |
| person.identifier.rid | I-8470-2012 | |
| person.identifier.scopus-author-id | 23569169900 | |
| person.identifier.scopus-author-id | 7102331969 | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | por |
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| relation.isAuthorOfPublication.latestForDiscovery | 7d93be47-8dc4-4413-9304-5b978773d3bb |