Publication
Phytochemical profiles and inhibitory effect on free radical-induced human erythrocyte damage of Dracaena draco leaf: A potential novel antioxidant agent
| dc.contributor.author | Santos, Rui P. | |
| dc.contributor.author | Mendes, Lídia S. | |
| dc.contributor.author | Silva, Branca M. | |
| dc.contributor.author | Pinho, P. Guedes de | |
| dc.contributor.author | Valentão, Patrícia | |
| dc.contributor.author | Andrade, Paula B. | |
| dc.contributor.author | Pereira, J.A. | |
| dc.contributor.author | Carvalho, Márcia | |
| dc.date.accessioned | 2011-01-14T14:21:10Z | |
| dc.date.available | 2011-01-14T14:21:10Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | The present study reports for the first time the metabolite profile and antioxidant activity of aqueous extract obtained from Dracaena draco L. leaf. Volatiles profile was determined by HS-SPME/GC-IT-MS, with 34 compounds being identified, distributed by distinct chemical classes: 2 alcohols, 5 aldehydes, 16 carotenoid derivatives and 8 terpenic compounds. Carotenoid derivative compounds constituted the most abundant class in leaf (representing 45% of total identified compounds). Phenolics profile was determined by HPLC/DAD and 9 constituents were identified: 2 hydroxycinnamic acid derivatives – 5- O-caffeoylquinic and 3,5-O-dicaffeoylquinic acids; 4 hydroxycinnamic acids – caffeic, p-coumaric, ferulic and sinapic acids and 3 flavonol glycosides – quercetin-3-O-rutinoside, kaempferol-3-O-glucoside and kaempferol-3-O-rutinoside. The most abundant phenolic compound is quercetin-3-O-rutinoside (representing 50.2% of total polyphenols). Organic acids composition was also characterised, by HPLC–UV and oxalic, citric, malic and fumaric acids were determined. Oxalic and citric acids were present in higher amounts (representing 47%, each). The antioxidant potential of this material was assessed by the ability to protect against free radical-induced biomembrane damage, using human erythrocyte as in vitro model. Leaf extract strongly protected the erythrocyte membrane from haemolysis (IC50 of 39 ± 11 lg/ml), in a time- and concentration-dependent manner. This is the first report showing that D. draco leaf is a promising antioxidant agent. | por |
| dc.identifier.citation | Santos, R.P.; Mendes, L.S.; Silva, B.M.; Guedes de Pinho, P.; Valentão, P.; Andrade, P.B.; Pereira, J. A.; Carvalho, M. (2011). Phytochemical profiles and inhibitory effect on free radical-induced human erythrocyte damage of Dracaena draco leaf: A potential novel antioxidant agent. Food Chemistry. ISSN 0308-8146. 124:3, p. 927-934. | por |
| dc.identifier.doi | 10.1016/j.foodchem.2010.07.021 | |
| dc.identifier.issn | 0308-8146 | |
| dc.identifier.uri | http://hdl.handle.net/10198/3124 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Dracaena draco leaf | por |
| dc.subject | Volatiles | por |
| dc.subject | Polyphenols | por |
| dc.subject | Organic acids | por |
| dc.subject | Haemolysis inhibition | por |
| dc.subject | Antioxidant activity | por |
| dc.title | Phytochemical profiles and inhibitory effect on free radical-induced human erythrocyte damage of Dracaena draco leaf: A potential novel antioxidant agent | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 934 | por |
| oaire.citation.issue | 124 | por |
| oaire.citation.startPage | 927 | por |
| oaire.citation.title | Food Chemistry | por |
| person.familyName | Pereira | |
| person.givenName | José Alberto | |
| person.identifier.ciencia-id | 611F-80B2-A7C1 | |
| person.identifier.orcid | 0000-0002-2260-0600 | |
| person.identifier.rid | L-6798-2014 | |
| person.identifier.scopus-author-id | 57204366348 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 7932162e-a2da-4913-b00d-17babbe51857 | |
| relation.isAuthorOfPublication.latestForDiscovery | 7932162e-a2da-4913-b00d-17babbe51857 |