Repository logo
 
Publication

Evaluation of the ability to form biofilms in KPC-producing and ESBL-producing Klebsiella pneumoniae isolated from clinical samples

2023Journal article Open access
Simple item page
dc.contributor.authorSabenca, Carolina
dc.contributor.authorCosta, Eliana
dc.contributor.authorSousa, Sara
dc.contributor.authorBarros, Lillian
dc.contributor.authorOliveira, Ana M.P.
dc.contributor.authorRamos, Sonia
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorTorres, Carmen
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2011-06-21T08:38:49Z
dc.date.available2011-06-21T08:38:49Z
dc.date.issued2023
dc.date.updated2011-06-18T15:12:13Z
dc.description.abstractThe appearance of Klebsiella pneumoniae strains producing extended-spectrum beta-lactamase (ESBL), and carbapenemase (KPC) has turned into a significant public health issue. ESBL- and KPC-producing K. pneumoniae's ability to form biofilms is a significant concern as it can promote the spread of antibiotic resistance and prolong infections in healthcare facilities. A total of 45 K. pneumoniae strains were isolated from human infections. Antibiograms were performed for 17 antibiotics, ESBL production was tested by Etest ESBL PM/PML, a rapid test was used to detect KPC carbapenemases, and resistance genes were detected by PCR. Biofilm production was detected by the microtiter plate method. A total of 73% of multidrug resistance was found, with the highest resistance rates to ampicillin, trimethoprim-sulfamethoxazole, cefotaxime, amoxicillin-clavulanic acid, and aztreonam. Simultaneously, the most effective antibiotics were tetracycline and amikacin. bla(CTX-M), bla(TEM), bla(SHV), aac(3)-II, aadA1, tetA, cmlA, catA, gyrA, gyrB, parC, sul1, sul2, sul3, bla(KPC), bla(OXA), and bla(PER) genes were detected. Biofilm production showed that 80% of K. pneumoniae strains were biofilm producers. Most ESBL- and KPC-producing isolates were weak biofilm producers (40.0% and 60.0%, respectively). There was no correlation between the ability to form stronger biofilms and the presence of ESBL and KPC enzymes in K. pneumoniae isolates.
dc.description.sponsorshipThis work was supported by the projects UIDP/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT).
dc.identifier.citationSabenca, Carolina; Costa, Eliana; Sousa, Sara; Barros, Lillian; Oliveira, Ana M.P.; Ramos, Sonia; Igrejas, Gilberto; Torres, Carmen; Poeta, Patrícia (2023). Evaluation of the ability to form biofilms in KPC-producing and ESBL-producing Klebsiella pneumoniae isolated from clinical samples. Antibiotics. ISSN 2079-6382. 12:7, p. 1-13por
dc.identifier.doi10.3390/antibiotics12071143
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10198/5277
dc.language.isoengpor
dc.publisherMDPIpor
dc.subjectKlebsiella pneumoniaepor
dc.subjectextended-spectrum β-lactamase (ESBL)por
dc.subjectKlebsiella pneumoniae carbapenemase (KPC)
dc.subjectBiofilm
dc.subjectAntimicrobial resistance
dc.titleEvaluation of the ability to form biofilms in KPC-producing and ESBL-producing Klebsiella pneumoniae isolated from clinical samplespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleAntibioticspor
person.familyNameBarros
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35236343600
rcaap.rightsopenAccesspor
rcaap.typearticle
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublication.latestForDiscovery3af07ffe-f914-48ba-a5d5-efcf70fdce01

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation of the ability to form biofilms.pdf
Size:
490.35 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus