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Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction

2018Journal article Open access
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dc.contributor.authorSantos, Klebson S.
dc.contributor.authorBarbosa, Andriele M.
dc.contributor.authorFreitas, Victor
dc.contributor.authorMuniz, Ana Veruska C.S.
dc.contributor.authorMendonça, Marcelo C.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorFranceschi, Elton
dc.contributor.authorPadilha, Francine F.
dc.contributor.authorOliveira, Beatriz
dc.contributor.authorDariva, Cláudio
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2019-02-04T17:08:48Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2019-02-04T17:08:48Z
dc.date.issued2018
dc.description.abstractAzadirachta indica A. Juss (neem) extracts have been used in pharmaceutical applications as antitumor agents, due to their terpenes and phenolic compounds. To obtain extracts from neem leaves with potential antiproliferative effect, a sequential process of pressurized liquid extraction was carried out in a fixed bed extractor at 25◦C and 100 bar, using hexane (SH), ethyl acetate (SEA), and ethanol (SE)assolvents. Extractions using only ethanol(EE) was also conducted to compare the characteristics of the fractionated extracts. The results obtained by liquid chromatography-electrospray ionization mass spectrometry suggested a higher concentration of terpenes in the SEA extract in comparison to SH, SE, and EE extracts. Therefore, antiproliferative activity showed that SEA extracts were the most efficient inhibitor to human tumor cells MCF-7, NCI-H460, HeLa, and HepG2. Hepatocellular cells were more resistant to SH, SEA, SE, and EE compared to breast, lung, hepatocellular, and cervical malignant cells. Neem fractioned extracts obtained in the present study seem to be more selective for malignant cells compared to the non-tumor cells.en_EN
dc.description.sponsorshipKlebson Silva Santos thanks CAPES (Process: PDSE 99999.003409/15-5) for the financial support during his Ph.D. studies in Portugal. Authors are grateful to CAPES, and FAPITEC (EDITAL CAPES/FAPITEC Nº 11/2016–PROEF/Processo de AUXPE 88881.157437/2017-01) for the financial support. The study was also carried out with financial support from FEDER, under the Partnership Agreement PT2020.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationSantos, Klebson S.; Barbosa, Andriele M.; Freitas, Victor; Muniz, Ana Veruska C.S.; Mendonça, Marcelo C.; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Franceschi, Elton; Padilha, Francine F.; Oliveira, M.B.P.P.; Dariva, Cláudio (2018). Antiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extraction. Pharmaceuticals. ISSN 14248247. 11:3en_EN
dc.identifier.doi10.3390/ph11030076en_EN
dc.identifier.urihttp://hdl.handle.net/10198/18725
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationPDSE99999.003409/15-5
dc.subjectAntiproliferative activityen_EN
dc.subjectNeem leavesen_EN
dc.subjectSequential pressurized liquid extractionen_EN
dc.titleAntiproliferative activity of neem leaf extracts obtained by a sequential pressurized liquid extractionen_EN
dc.typejournal article
dspace.entity.typePublication
person.familyNameCalhelha
person.familyNameFerreira
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.identifier144781
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication2d5d1a41-7561-4a01-871c-b4c97da35053
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscoverybd0d1537-2e03-41fb-b27a-140af9c35db8

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