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A Spectrochemically Driven Study: Identifying Phenolic-Rich Extracts from Helichrysum stoechas, Lavandula pedunculata, and Thymus mastichina with Potential to Revert Skin Aging Effects

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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg12:Produção e Consumo Sustentáveis
dc.contributor.authorMarques, Mário Pedro
dc.contributor.authorLandim, Euclides
dc.contributor.authorVarela, Carla
dc.contributor.authorCosta, Ricardo M. F. da
dc.contributor.authorMarques, Joana
dc.contributor.authorCarvalho, Luís A. E. Batista de
dc.contributor.authorSilva, Ana
dc.contributor.authorCruz, Maria Teresa
dc.contributor.authorAndré, Rebeca
dc.contributor.authorRijo, Patrícia
dc.contributor.authorDias, Maria Inês
dc.contributor.authorCarvalho, Aida
dc.contributor.authorOliveira, Paulo J.
dc.contributor.authorCabral, Célia
dc.date.accessioned2026-01-15T16:17:43Z
dc.date.available2026-01-15T16:17:43Z
dc.date.issued2025
dc.description.abstractPlants inhabiting mediterranean-influenced climatic zones, like Helichrysum stoechas (L.) Moench subsp. stoechas, Lavandula pedunculata (Mill.) Cav., and Thymus mastichina (L.) L. subsp. mastichina, have been scarcely investigated regarding their richness in phenolic compounds, herein explored as sources of skin anti-aging compounds. In this investigation, Fourier transform infrared spectroscopy (FTIR) in attenuated total reflectance (ATR) mode and high-performance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) were employed to chemically characterize the hydroethanolic extracts (HEs), and their cell-free antioxidant potential was screened. Thereafter, non-toxic concentrations of HEs were determined in human skin cells using Alamar blue® and Sulforhodamine B assays. The cytoprotective and antioxidant effects of HEs were assessed in tert-butyl hydroperoxide-stimulated fibroblasts, their anti-inflammatory potential was studied in lipopolysaccharide-injured macrophages, and enzymatic inhibition assays were performed. Notably, the irritant effects of HEs were tested according to Test Guideline No. 439 of the Organization for Economic Co-operation and Development (OECD). The major compounds identified in the T. mastichina and L. pedunculata HEs were rosmarinic and salvianolic acid derivatives, while H. stoechas HE was mainly composed of caffeoyl and feruloyl derivatives, and O-glycosylated flavonoids. T. mastichina (≤0.4 mg/mL) exhibited significant cytoprotective, anti-inflammatory, and antioxidant effects, as well as remarkable anti-hyaluronidase activity. Shedding light on the quantitative and qualitative chemical picture of these HEs highlighted T. mastichina as a promising candidate to target skin aging effects, which correlates with its phenolic content. Further investigation is warranted regarding its anti-aging pharmacological activity, which could lead to the development of plant-based skin anti-aging products.eng
dc.description.sponsorshipThe authors express their thanks to Rosa Pinho for the taxonomic identification of plants used in this study, and to Lísia Lopes, who captured the photographs presented in the graphical abstract of this manuscript, under the FCT-funded project CôaMedPlants.
dc.identifier.citationMarques, Mario Pedro; Landim, Euclides; Varela, Carla; Da Costa, Ricardo M. F.; Marques, Joana; Batista de Carvalho, Luis A. E.; Silva, Ana; Cruz, Maria Teresa; Andre, Rebeca; Rijo, Patricia; Dias, Maria Ines; Carvalho, Aida; Oliveira, Paulo J.; Cabral, Celia (2025). A Spectrochemically Driven Study: Identifying Phenolic-Rich Extracts from Helichrysum stoechas, Lavandula pedunculata, and Thymus mastichina with Potential to Revert Skin Aging Effects. Pharmaceuticals. ISSN 1424-8247. 18:12, p. 1-31
dc.identifier.doi10.3390/ph18121889
dc.identifier.issn1424-8247
dc.identifier.urihttp://hdl.handle.net/10198/35495
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationCôaMedPlant
dc.relation.ispartofPharmaceuticals
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectPlant extracts
dc.subjectPhenolic compounds
dc.subjectSkin aging
dc.subjectFTIR-ATR
dc.subjectHPLC-DAD-ESI/MSn
dc.titleA Spectrochemically Driven Study: Identifying Phenolic-Rich Extracts from Helichrysum stoechas, Lavandula pedunculata, and Thymus mastichina with Potential to Revert Skin Aging Effectseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.titlePharmaceutical Biology
oaire.citation.volume18
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDias
person.givenNameMaria Inês
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.orcid0000-0001-8744-7814
person.identifier.ridM-8242-2013
person.identifier.scopus-author-id54388787000
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublication.latestForDiscovery7ca8432b-e7f4-4116-a56e-9eba0d4403bd

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