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Chromatographic analysis of nutritional and bioactive compounds in vegetative parts of Fragaria vesca L. obtained by in vitro culture

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dc.contributor.authorDias, Maria Inês
dc.contributor.authorBarros, Lillian
dc.contributor.authorSousa, Maria João
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2017-12-21T09:46:59Z
dc.date.available2017-12-21T09:46:59Z
dc.date.issued2017
dc.description.abstractFragaria vesca L. is mainly consumed and appreciated by its sweet small fruits. However, the vegetative parts have been described as important sources of macro and micronutrients as also phenolic compounds (procyanidins and hydroxycinnamoyl and ellagic acid derivatives) [1,2]. The growing demand for natural products with nutritional value and, simultaneously, bioactive properties requires innovation in how to obtain these products, namely protecting the wild populations from where they are collected avoiding direct competition with human food stuff cultures [3]. Cell and tissue culture emerges as viable technique to produce secondary metabolites, being a sustainable and ecological alternative to obtain bioactive compounds that can be applied in pharmaceutical/medical and/or food industry (endorsed by FAO) [5]. Herein, the vegetative parts of F. vesca were obtained by in vitro culture and characterized in terms of macronutrients (AOAC methods), fatty acids (GC-FID), sugars (HPLC-RI), organic acids (HPLC-DAD), tocopherols (HPLC-Fluorescence), and phenolic compounds (HPLC-DADESI/ MS). The antioxidant activity was also evaluated. The studies were carried out with lyophilized material and hydromethanolic and aqueous (infusions and decoctions) extracts. In order to establish the in vitro culture, a four week interval was followed for micropropagation In each one, the vegetative parts were collected and characterized, presenting higher contents of proteins, fatty acids, sugars, organic acids (including ascorbic acid) and tocopherols (mainly α-tocopherol) than the samples obtained from wild vegetative parts collected in field and previously studied by our research group [2]. Furthermore, the hydromethanolic extracts of the obtained in vitro samples also revealed higher antioxidant activity that the F. vesca samples collected in field [4]. On the contrary and despite the similarity between the phenolic profile of both samples, lower concentrations were detected in the in vitro cultured F. vesca. Further studies are required to understand how to increase the concentration of phenolic compounds by elicitation of the in vitro culture. Within this work, it was intended to explore the in vitro culture as a biotechnological tool for the obtention of high value phytochemicals with application in different industrial sectors.pt_PT
dc.description.sponsorshipFoundation for Science and Technology (FCT) and FEDER (Programme PT2020) for the financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract. To the Interreg Espanha-Portugal (0377_Iberphenol_6_E) project. To GIP-USAL (Governo Espanhol through the project AGL2015-64522-C2-2-R).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDias, Maria Inês; Barros, Lillian; Sousa, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2017). Chromatographic analysis of nutritional and bioactive compounds in vegetative parts of Fragaria vesca L. obtained by in vitro culture. In 10º Encontro Nacional de Cromatografia. Bragança. ISBN 978-972-745-234-7pt_PT
dc.identifier.isbn978-972-745-234-7
dc.identifier.urihttp://hdl.handle.net/10198/14792
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleChromatographic analysis of nutritional and bioactive compounds in vegetative parts of Fragaria vesca L. obtained by in vitro culturept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.citation.conferencePlaceBragança, Portugalpt_PT
oaire.citation.title10º Encontro Nacional de Cromatografiapt_PT
oaire.fundingStream5876
person.familyNameDias
person.familyNameBarros
person.familyNameSousa
person.familyNameFerreira
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameMaria João
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-idD91E-3571-3979
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-9946-4926
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id55230282700
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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