Publication
Cordyceps militaris (L.) Link fruiting body reduces the growth of a non-small cell lung cancer cell line by increasing cellular levels of p53 and p21
| dc.contributor.author | Bizarro, Ana | |
| dc.contributor.author | Ferreira, Isabel C.F.R. | |
| dc.contributor.author | Soković, Marina | |
| dc.contributor.author | Van Griensven, Leo J.L.D. | |
| dc.contributor.author | Sousa, Diana | |
| dc.contributor.author | Vasconcelos, M. Helena | |
| dc.contributor.author | Lima, Raquel T. | |
| dc.date.accessioned | 2015-11-30T15:14:20Z | |
| dc.date.available | 2015-11-30T15:14:20Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Cordyceps militaris (L.) Link, an edible entomopathogenic fungus widely used in traditional Chinese medicine, has numerous potential medicinal properties including antitumor activity. The methanolic extract of C. militaris fruiting body was recently shown to have tumor cell growth inhibitory activity in several human tumor cell lines. Nonetheless, the mechanism of action involved is still not known. This work aimed at further studying the effect of the methanolic extract of C. militaris regarding its antitumor mechanism of action, using the non-small cell lung cancer cell line (NCI-H460) as a model. Results showed that treatment with the extract decreased cellular proliferation, induced cell cycle arrest at G0/G1 and increased apoptosis. In addition, the extract increased the levels of p53 and p21. Moreover, an increase in p-H2A.X and 53BP1 levels, together with an increase in the number of 53BP1 foci/cell (all indicative of DNA damage), were also observed after treatment with the extract. This work suggests that this extract affected NCI-H460 cellular viability through a mechanism involving DNA damage and p53 activation. This further supports the potential of this extract as a source of bioactive compounds, which may be used in anticancer strategies. | pt_PT |
| dc.description.sponsorship | IPATIMUP integrates the i3S Research Unit, which is partially supported by FCT, the Portuguese Foundation for Science and Technology. This work is funded by FEDER funds through the Operational Programme for Competitiveness Factors (COMPETE) and National Funds through the FCT, Foundation for Science and Technology, under the projects “PEst-C/SAU/LA0003/2013” and FCOMP-01-0124-FEDER-015752 (ref FCT PTDC/SAU-FAR/110848/2009). The authors also thank: FCT for the grant to R.T.L. (SFRH/BPD/68787/2010) and QREN for the grant of D.S. (NORTE-07-0124-FEDER-000023). | pt_PT |
| dc.identifier.citation | Bizarro, Ana; Ferreira, Isabel C.F.R.; Soković, Marina; Van Griensven, Leo J.L.D.; Sousa, Diana; Vasconcelos, M. Helena; Lima, Raquel T. (2015). Cordyceps militaris (L.) link fruiting body reduces NCI-H460 cellular viability through a mechanism involving p53 and p21. Molecules. ISSN 1420-3049. 20:8, p. 13927-13940 | pt_PT |
| dc.identifier.doi | 10.3390/molecules200813927 | pt_PT |
| dc.identifier.issn | 1420-3049 | |
| dc.identifier.uri | http://hdl.handle.net/10198/12457 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.relation | NORTE-07-0124-FEDER-000023 | pt_PT |
| dc.relation | Functional, molecular and pharmacological studies of p53 family proteins: from yeast to human cells | |
| dc.relation | SMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Cordyceps militaris methanolic extract | pt_PT |
| dc.subject | p53 | pt_PT |
| dc.subject | p21 | pt_PT |
| dc.subject | Cell cycle arrest | pt_PT |
| dc.subject | DNA damage | pt_PT |
| dc.title | Cordyceps militaris (L.) Link fruiting body reduces the growth of a non-small cell lung cancer cell line by increasing cellular levels of p53 and p21 | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Functional, molecular and pharmacological studies of p53 family proteins: from yeast to human cells | |
| oaire.awardTitle | SMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/Incentivo%2FSAU%2FLA0003%2F2013/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-FAR%2F110848%2F2009/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68787%2F2010/PT | |
| oaire.citation.endPage | 13940 | pt_PT |
| oaire.citation.startPage | 13927 | pt_PT |
| oaire.citation.title | Molecules | pt_PT |
| oaire.citation.volume | 20 | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 5876-PPCDTI | |
| person.familyName | Ferreira | |
| person.givenName | Isabel C.F.R. | |
| person.identifier | 144781 | |
| person.identifier.ciencia-id | 9418-CF95-9919 | |
| person.identifier.orcid | 0000-0003-4910-4882 | |
| person.identifier.rid | E-8500-2013 | |
| person.identifier.scopus-author-id | 36868826600 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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