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Optimization of high pressure bioactive compounds extraction from pansies (Viola × wittrockiana) by response surface methodology

dc.contributor.authorFernandes, Luana
dc.contributor.authorCasal, Susana
dc.contributor.authorPereira, J.A.
dc.contributor.authorRamalhosa, Elsa
dc.contributor.authorSaraiva, Jorge A.
dc.date.accessioned2017-09-11T14:50:16Z
dc.date.available2017-09-11T14:50:16Z
dc.date.issued2017
dc.description.abstractResponse surface methodology (RSM) was employed for the first time to optimize high pressure extraction (HPE) conditions of bioactive compounds from pansies, namely: pressure (X1: 0–500 MPa), time (X2: 5–15 min) and ethanol concentration (X3: 0–100%). Consistent fittings using second-order polynomial models were obtained for flavonoids, tannins, anthocyanins, total reducing capacity (TRC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The optimum extraction conditions based on combination responses for TRC, tannins and anthocyanins were: X1 = 384 MPa, X2 = 15 min and X3 = 35% (v/v) ethanol, shortening the extraction time when compared to the classic method of stirring (approx. 24 h). When the optimum extraction conditions were applied, 65.1 mg of TRC, 42.8 mg of tannins and 56.15 mg of anthocyanins/g dried flower were obtained. Thus, HPE has shown to be a promising technique to extract bioactive compounds from pansies, by reducing the extraction time and by using green solvents (ethanol and water), for application in diverse industrial fields.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, Luana; Casal, Susana; Pereira, J.A.; Ramalhosa, Elsa; Saraiva, Jorge (2017). Optimization of high pressure bioactive compounds extraction from pansies (Viola × wittrockiana) by response surface methodology. High Pressure Research. ISSN 0895-7959. 37:3, p. 415-429pt_PT
dc.identifier.doi10.1080/08957959.2017.1347925pt_PT
dc.identifier.issn0895-7959
dc.identifier.urihttp://hdl.handle.net/10198/14467
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPansies flowerspt_PT
dc.subjectHigh pressure extractionpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectBioactive compoundspt_PT
dc.subjectResponse surface methodologypt_PT
dc.titleOptimization of high pressure bioactive compounds extraction from pansies (Viola × wittrockiana) by response surface methodologypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F95853%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F00062%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.citation.endPage429pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage415pt_PT
oaire.citation.titleHigh Pressure Researchpt_PT
oaire.citation.volume37pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameFernandes
person.familyNamePereira
person.familyNameRamalhosa
person.givenNameLuana
person.givenNameJosé Alberto
person.givenNameElsa
person.identifier.ciencia-id1219-7C60-1311
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-id1A1D-FC05-A05D
person.identifier.orcid0000-0001-9377-2453
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0003-2503-9705
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id6602978189
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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