Publication
Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
| dc.contributor.author | Pinto, Ricardo | |
| dc.contributor.author | Amaral, António L. | |
| dc.contributor.author | Ferreira, Eugénio C. | |
| dc.contributor.author | Mota, Manuel | |
| dc.contributor.author | Vilanova, Manuel | |
| dc.contributor.author | Ruel, Katia | |
| dc.contributor.author | Gama, Miguel | |
| dc.date.accessioned | 2018-06-08T14:42:09Z | |
| dc.date.available | 2018-06-08T14:42:09Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results: For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 x 10(-13) mol/mm(2) was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion: It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20). | pt_PT |
| dc.description.sponsorship | Ricardo Pinto was supported by a grant from the Fundação para a Ciência e a Tecnologia (FCT) SFRH/BD/6934/2001. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Pinto, Ricardo; Amaral, Antonio L.; Ferreira, Eugenio C.; Mota, Manuel; Vilanova, Manuel; Ruel, Katia; Gama, Miguel (2008). Quantification of the CBD-FITC conjugates surface coating on cellulose fibres. BMC Biotechnology. ISSN 1472-6750. 8, p. 1-7 | pt_PT |
| dc.identifier.doi | 10.1186/1472-6750-8-1 | pt_PT |
| dc.identifier.issn | 1472-6750 | |
| dc.identifier.uri | http://hdl.handle.net/10198/17686 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.title | Quantification of the CBD-FITC conjugates surface coating on cellulose fibres | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F6934%2F2001/PT | |
| oaire.citation.endPage | 7 | pt_PT |
| oaire.citation.issue | 1 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| oaire.citation.title | BMC Biotechnology | pt_PT |
| oaire.citation.volume | 8 | pt_PT |
| oaire.fundingStream | SFRH | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 55262048-0716-49ea-81df-8eaeebe84c19 | |
| relation.isProjectOfPublication.latestForDiscovery | 55262048-0716-49ea-81df-8eaeebe84c19 |